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Genotype-independent and enhanced in planta Agrobacterium tumefaciens-mediated genetic transformation of peanut [Arachis hypogaea (L.)]
Agrobacterium infection and regeneration of the putatively transformed plant from the explant remains arduous for some crop species like peanut. Henceforth, a competent and reproducible in planta genetic transformation protocol is established for peanut cv. CO7 by standardizing various factors such...
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| Published in: | 3 Biotech 2018-04, Vol.8 (4), p.202-202, Article 202 |
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| creator | Karthik, Sivabalan Pavan, Gadamchetty Sathish, Selvam Siva, Ramamoorthy Kumar, Periyasamy Suresh Manickavasagam, Markandan |
| description | Agrobacterium
infection and regeneration of the putatively transformed plant from the explant remains arduous for some crop species like peanut. Henceforth, a competent and reproducible in planta genetic transformation protocol is established for peanut cv. CO7 by standardizing various factors such as pre-culture duration, acetosyringone concentration, duration of co-cultivation, sonication and vacuum infiltration. In the present investigation,
Agrobacterium tumefaciens
strain EHA105 harboring the binary vector pCAMBIA1301–
bar
was used for transformation. The two-stage selection was carried out using 4 and 250 mg l
−1
BASTA
®
to completely eliminate the chimeric and non-transformed plants. The transgene integration into plant genome was evaluated by GUS histochemical assay, polymerase chain reaction (PCR), and Southern blot hybridization. Among the various combinations and concentrations analyzed, highest transformation efficiency was obtained when the 2-day pre-cultured explants were subjected to sonication for 6 min and vacuum infiltrated for 3 min in
Agrobacterium
suspension, and co-cultivated on MS medium supplemented with 150 µM acetosyringone for 3 days. The fidelity of the standardized in planta transformation method was assessed in five peanut cultivars and all the cultivars responded positively with a transformation efficiency ranging from minimum 31.3% (with cv. CO6) to maximum 38.6% (with cv. TMV7). The in planta transformation method optimized in this study could be beneficial to develop superior peanut cultivars with desirable genetic traits. |
| doi_str_mv | 10.1007/s13205-018-1231-1 |
| format | article |
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infection and regeneration of the putatively transformed plant from the explant remains arduous for some crop species like peanut. Henceforth, a competent and reproducible in planta genetic transformation protocol is established for peanut cv. CO7 by standardizing various factors such as pre-culture duration, acetosyringone concentration, duration of co-cultivation, sonication and vacuum infiltration. In the present investigation,
Agrobacterium tumefaciens
strain EHA105 harboring the binary vector pCAMBIA1301–
bar
was used for transformation. The two-stage selection was carried out using 4 and 250 mg l
−1
BASTA
®
to completely eliminate the chimeric and non-transformed plants. The transgene integration into plant genome was evaluated by GUS histochemical assay, polymerase chain reaction (PCR), and Southern blot hybridization. Among the various combinations and concentrations analyzed, highest transformation efficiency was obtained when the 2-day pre-cultured explants were subjected to sonication for 6 min and vacuum infiltrated for 3 min in
Agrobacterium
suspension, and co-cultivated on MS medium supplemented with 150 µM acetosyringone for 3 days. The fidelity of the standardized in planta transformation method was assessed in five peanut cultivars and all the cultivars responded positively with a transformation efficiency ranging from minimum 31.3% (with cv. CO6) to maximum 38.6% (with cv. TMV7). The in planta transformation method optimized in this study could be beneficial to develop superior peanut cultivars with desirable genetic traits.</description><identifier>ISSN: 2190-572X</identifier><identifier>EISSN: 2190-5738</identifier><identifier>DOI: 10.1007/s13205-018-1231-1</identifier><identifier>PMID: 29607283</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acetosyringone ; Agriculture ; Agrobacterium ; Agrobacterium radiobacter ; Agrobacterium tumefaciens ; Arachis hypogaea ; Bioinformatics ; Biomaterials ; Biotechnology ; Cancer Research ; Chemistry ; Chemistry and Materials Science ; chimerism ; coculture ; crops ; Cultivars ; Cultivation ; Explants ; genetic traits ; Genetic transformation ; Genomes ; Genotypes ; Hybridization ; Infiltration ; Original ; Original Article ; Peanuts ; Polymerase chain reaction ; Regeneration ; Sonication ; Southern blotting ; Stem Cells ; transgenes ; Transgenic plants ; Vacuum</subject><ispartof>3 Biotech, 2018-04, Vol.8 (4), p.202-202, Article 202</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>3 Biotech is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-bd57ce9c8885a4f4aae96ccf9f6387b110dd80ffd0e2c15c4fb41118d1ac2df93</citedby><cites>FETCH-LOGICAL-c503t-bd57ce9c8885a4f4aae96ccf9f6387b110dd80ffd0e2c15c4fb41118d1ac2df93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874222/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874222/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29607283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karthik, Sivabalan</creatorcontrib><creatorcontrib>Pavan, Gadamchetty</creatorcontrib><creatorcontrib>Sathish, Selvam</creatorcontrib><creatorcontrib>Siva, Ramamoorthy</creatorcontrib><creatorcontrib>Kumar, Periyasamy Suresh</creatorcontrib><creatorcontrib>Manickavasagam, Markandan</creatorcontrib><title>Genotype-independent and enhanced in planta Agrobacterium tumefaciens-mediated genetic transformation of peanut [Arachis hypogaea (L.)]</title><title>3 Biotech</title><addtitle>3 Biotech</addtitle><addtitle>3 Biotech</addtitle><description>Agrobacterium
infection and regeneration of the putatively transformed plant from the explant remains arduous for some crop species like peanut. Henceforth, a competent and reproducible in planta genetic transformation protocol is established for peanut cv. CO7 by standardizing various factors such as pre-culture duration, acetosyringone concentration, duration of co-cultivation, sonication and vacuum infiltration. In the present investigation,
Agrobacterium tumefaciens
strain EHA105 harboring the binary vector pCAMBIA1301–
bar
was used for transformation. The two-stage selection was carried out using 4 and 250 mg l
−1
BASTA
®
to completely eliminate the chimeric and non-transformed plants. The transgene integration into plant genome was evaluated by GUS histochemical assay, polymerase chain reaction (PCR), and Southern blot hybridization. Among the various combinations and concentrations analyzed, highest transformation efficiency was obtained when the 2-day pre-cultured explants were subjected to sonication for 6 min and vacuum infiltrated for 3 min in
Agrobacterium
suspension, and co-cultivated on MS medium supplemented with 150 µM acetosyringone for 3 days. The fidelity of the standardized in planta transformation method was assessed in five peanut cultivars and all the cultivars responded positively with a transformation efficiency ranging from minimum 31.3% (with cv. CO6) to maximum 38.6% (with cv. TMV7). The in planta transformation method optimized in this study could be beneficial to develop superior peanut cultivars with desirable genetic traits.</description><subject>Acetosyringone</subject><subject>Agriculture</subject><subject>Agrobacterium</subject><subject>Agrobacterium radiobacter</subject><subject>Agrobacterium tumefaciens</subject><subject>Arachis hypogaea</subject><subject>Bioinformatics</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Cancer Research</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>chimerism</subject><subject>coculture</subject><subject>crops</subject><subject>Cultivars</subject><subject>Cultivation</subject><subject>Explants</subject><subject>genetic traits</subject><subject>Genetic transformation</subject><subject>Genomes</subject><subject>Genotypes</subject><subject>Hybridization</subject><subject>Infiltration</subject><subject>Original</subject><subject>Original Article</subject><subject>Peanuts</subject><subject>Polymerase chain reaction</subject><subject>Regeneration</subject><subject>Sonication</subject><subject>Southern blotting</subject><subject>Stem Cells</subject><subject>transgenes</subject><subject>Transgenic plants</subject><subject>Vacuum</subject><issn>2190-572X</issn><issn>2190-5738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqNkk2LFDEQhhtR3GXdH-BFAl7WQ6-p9Ff6IgyLuwoDXhQEkVCdrsxkmU7aJC3ML_Bvb5ZZxw8QzCEJ1FNvqlJvUTwHfgmcd68jVII3JQdZgqighEfFqYCel01XycfHu_h8UpzHeMvzaqDpgT8tTkTf8k7I6rT4cUPOp_1MpXUjzZQ3lxi6kZHbotM0MuvYvEOXkK02wQ-oEwW7TCwtExnUllwsJxotpgxvyFGymqWALhofJkzWO-YNmwndktiXVUC9tZFt97PfICG7WF---vqseGJwF-n84TwrPl2__Xj1rlx_uHl_tVqXuuFVKoex6TT1WkrZYG1qROpbrU1v2kp2AwAfR8mNGTkJDY2uzVADgBwBtRhNX50Vbw668zLkonXuNuBOzcFOGPbKo1V_Rpzdqo3_rhrZ1UKILHDxIBD8t4ViUpONmnb5h8gvUeWZVFJWTfs_qOBS9pnP6Mu_0Fu_BJd_IlPQizYPkmcKDpQOPsZA5lg3cHVvCnUwhcqmUPemUJBzXvze8DHjpwUyIA5AzCG3ofDr6X-r3gGGfsT2</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Karthik, Sivabalan</creator><creator>Pavan, Gadamchetty</creator><creator>Sathish, Selvam</creator><creator>Siva, Ramamoorthy</creator><creator>Kumar, Periyasamy Suresh</creator><creator>Manickavasagam, Markandan</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20180401</creationdate><title>Genotype-independent and enhanced in planta Agrobacterium tumefaciens-mediated genetic transformation of peanut [Arachis hypogaea (L.)]</title><author>Karthik, Sivabalan ; Pavan, Gadamchetty ; Sathish, Selvam ; Siva, Ramamoorthy ; Kumar, Periyasamy Suresh ; Manickavasagam, Markandan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-bd57ce9c8885a4f4aae96ccf9f6387b110dd80ffd0e2c15c4fb41118d1ac2df93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acetosyringone</topic><topic>Agriculture</topic><topic>Agrobacterium</topic><topic>Agrobacterium radiobacter</topic><topic>Agrobacterium tumefaciens</topic><topic>Arachis hypogaea</topic><topic>Bioinformatics</topic><topic>Biomaterials</topic><topic>Biotechnology</topic><topic>Cancer Research</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>chimerism</topic><topic>coculture</topic><topic>crops</topic><topic>Cultivars</topic><topic>Cultivation</topic><topic>Explants</topic><topic>genetic traits</topic><topic>Genetic transformation</topic><topic>Genomes</topic><topic>Genotypes</topic><topic>Hybridization</topic><topic>Infiltration</topic><topic>Original</topic><topic>Original Article</topic><topic>Peanuts</topic><topic>Polymerase chain reaction</topic><topic>Regeneration</topic><topic>Sonication</topic><topic>Southern blotting</topic><topic>Stem Cells</topic><topic>transgenes</topic><topic>Transgenic plants</topic><topic>Vacuum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karthik, Sivabalan</creatorcontrib><creatorcontrib>Pavan, Gadamchetty</creatorcontrib><creatorcontrib>Sathish, Selvam</creatorcontrib><creatorcontrib>Siva, Ramamoorthy</creatorcontrib><creatorcontrib>Kumar, Periyasamy Suresh</creatorcontrib><creatorcontrib>Manickavasagam, Markandan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>3 Biotech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karthik, Sivabalan</au><au>Pavan, Gadamchetty</au><au>Sathish, Selvam</au><au>Siva, Ramamoorthy</au><au>Kumar, Periyasamy Suresh</au><au>Manickavasagam, Markandan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotype-independent and enhanced in planta Agrobacterium tumefaciens-mediated genetic transformation of peanut [Arachis hypogaea (L.)]</atitle><jtitle>3 Biotech</jtitle><stitle>3 Biotech</stitle><addtitle>3 Biotech</addtitle><date>2018-04-01</date><risdate>2018</risdate><volume>8</volume><issue>4</issue><spage>202</spage><epage>202</epage><pages>202-202</pages><artnum>202</artnum><issn>2190-572X</issn><eissn>2190-5738</eissn><abstract>Agrobacterium
infection and regeneration of the putatively transformed plant from the explant remains arduous for some crop species like peanut. Henceforth, a competent and reproducible in planta genetic transformation protocol is established for peanut cv. CO7 by standardizing various factors such as pre-culture duration, acetosyringone concentration, duration of co-cultivation, sonication and vacuum infiltration. In the present investigation,
Agrobacterium tumefaciens
strain EHA105 harboring the binary vector pCAMBIA1301–
bar
was used for transformation. The two-stage selection was carried out using 4 and 250 mg l
−1
BASTA
®
to completely eliminate the chimeric and non-transformed plants. The transgene integration into plant genome was evaluated by GUS histochemical assay, polymerase chain reaction (PCR), and Southern blot hybridization. Among the various combinations and concentrations analyzed, highest transformation efficiency was obtained when the 2-day pre-cultured explants were subjected to sonication for 6 min and vacuum infiltrated for 3 min in
Agrobacterium
suspension, and co-cultivated on MS medium supplemented with 150 µM acetosyringone for 3 days. The fidelity of the standardized in planta transformation method was assessed in five peanut cultivars and all the cultivars responded positively with a transformation efficiency ranging from minimum 31.3% (with cv. CO6) to maximum 38.6% (with cv. TMV7). The in planta transformation method optimized in this study could be beneficial to develop superior peanut cultivars with desirable genetic traits.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29607283</pmid><doi>10.1007/s13205-018-1231-1</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | 3 Biotech, 2018-04, Vol.8 (4), p.202-202, Article 202 |
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| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5874222 |
| source | Springer Nature; PubMed Central |
| subjects | Acetosyringone Agriculture Agrobacterium Agrobacterium radiobacter Agrobacterium tumefaciens Arachis hypogaea Bioinformatics Biomaterials Biotechnology Cancer Research Chemistry Chemistry and Materials Science chimerism coculture crops Cultivars Cultivation Explants genetic traits Genetic transformation Genomes Genotypes Hybridization Infiltration Original Original Article Peanuts Polymerase chain reaction Regeneration Sonication Southern blotting Stem Cells transgenes Transgenic plants Vacuum |
| title | Genotype-independent and enhanced in planta Agrobacterium tumefaciens-mediated genetic transformation of peanut [Arachis hypogaea (L.)] |
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