Preventive effect of Dioscorea japonica on squamous cell carcinoma of mouse skin involving down-regulation of prostaglandin E2 synthetic pathway

Hyperproduced prostaglandin E2 by cyclooxygenase-2 and microsomal prostaglandin E synthase-1 evokes several pathophysiological responses such as inflammation and carcinogenesis. Our recent study demonstrated that Dioscorea japonica extract suppressed the expression of cyclooxygenase-2 and microsomal...

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Bibliographic Details
Published in:Journal of Clinical Biochemistry and Nutrition 2018, Vol.62(2), pp.139-147
Main Authors: Tsukayama, Izumi, Toda, Keisuke, Takeda, Yasunori, Mega, Takuto, Tanaka, Mitsuki, Kawakami, Yuki, Takahashi, Yoshitaka, Kimoto, Masumi, Yamamoto, Kei, Miki, Yoshimi, Murakami, Makoto, Suzuki-Yamamoto, Toshiko
Format: Article
Language:English
Subjects:
Apoptosis
Carcinogenesis
Carcinogens
Cyclooxygenase-2
Cytokines
Dendritic cells
Dioscorea japonica
Hyperplasia
IL-1β
Immunoreactivity
Infiltration
Inflammation
Interleukin 6
Keratinocytes
Langerhans cells
Lung carcinoma
Metastases
Original
Prostaglandin D2 synthase
Prostaglandin E
Prostaglandin E2
Prostaglandin-E synthase
Rodents
Skin
Skin cancer
Squamous cell carcinoma
Topical application
Tumors
wild yam
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Summary:Hyperproduced prostaglandin E2 by cyclooxygenase-2 and microsomal prostaglandin E synthase-1 evokes several pathophysiological responses such as inflammation and carcinogenesis. Our recent study demonstrated that Dioscorea japonica extract suppressed the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 and induced apoptosis in lung carcinoma A549 cells. In the present study, we investigated the effects of Dioscorea japonica on squamous cell carcinoma of mouse skin. Dioscorea japonica feeding and Dioscorea japonica extract topical application suppressed the expression of cyclooxygenase-2, microsomal prostaglandin E synthase-1, interleukin-1β and interleukin-6 and inhibited tumor formation, hyperplasia and inflammatory cell infiltration. Immunohistochemical analyses showed the immunoreactivities of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 in tumor keratinocytes and stronger immunoreactivities of cyclooxygenase-2 and hematopoietic prostaglandin D synthase in epidermal dendritic cells (Langerhans cells). Treatment with Dioscorea japonica decreased the immunoreactivity of cyclooxygenase-2 and microsomal prostaglandin E synthase-1. These results indicate that Dioscorea japonica may have inhibitory effects on inflammation and carcinogenesis via suppression of the prostaglandin E2 synthetic pathway.
ISSN:0912-0009
1880-5086
DOI:10.3164/jcbn.17-54