The secretome from bovine mammosphere-derived cells (MDC) promotes angiogenesis, epithelial cell migration, and contains factors associated with defense and immunity

Treatment of bovine mastitis with intramammary antibiotics is common, yet several concerns exist including failed efficacy for individual hosts or pathogens and the inability of approved drugs to revert mastitis-induced tissue damage to healthy tissue capable of returning to full milk production. Th...

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Bibliographic Details
Published in:Scientific reports 2018-03, Vol.8 (1), p.5378-13, Article 5378
Main Authors: Ledet, Melissa M., Vasquez, Amy K., Rauner, Gat, Bichoupan, Allison A., Moroni, Paolo, Nydam, Daryl V., Van de Walle, Gerlinde R.
Format: Article
Language:English
Subjects:
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Angiogenesis
Antibiotics
Antimicrobial resistance
Cell adhesion & migration
Cell migration
Epithelial cells
Freezing
Humanities and Social Sciences
Mammary gland
Mastitis
Milk production
multidisciplinary
Public health
Science
Science (multidisciplinary)
Secretome
Thawing
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Summary:Treatment of bovine mastitis with intramammary antibiotics is common, yet several concerns exist including failed efficacy for individual hosts or pathogens and the inability of approved drugs to revert mastitis-induced tissue damage to healthy tissue capable of returning to full milk production. These issues, in addition to aspects of public health such as accidental antibiotic residues in saleable milk and the potential for antimicrobial resistance, support the need to find alternative therapies for this costly disease. This study shows that the secretome, or collective factors, produced by mammosphere-derived cells (MDC) promotes angiogenesis, epithelial cell migration, and contains proteins associated with immunity and defense; all of which are necessary for healing damaged mammary gland tissue. Furthermore, we found that the MDC secretome remains effective after freezing and thawing, enhancing its therapeutic potential. Our results provide a foundation for further characterization of the individual secreted factors and the rationale for using the MDC secretome as a complementary treatment for bovine mastitis.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-23770-z