Association of P53 gene polymorphism with gastric cancer in Northern Iran as a high-risk region

• Published in: Biomedical reports 2018-05, Vol.8 (5), p.433-438
• Main Authors: Hedayatizadeh-Omran, Akbar, Alizadeh-Navaei, Reza, Janbabaei, Ghasem, Omrani-Nava, Versa, Hasheminasab, Yahya, Amjadi, Omolbanin, Tehrani, Mohsen
• Format: Article
• Language: English
• Subjects: Age; Apoptosis; Breast cancer; Cancer; Cell cycle; Colorectal cancer; Deoxyribonucleic acid; DNA; DNA repair; Gastric cancer; Gender; Gene polymorphism; Genes; Genetic aspects; Genetic factors; Genetic polymorphisms; Genomes; Genotypes; Laboratories; Leukocytes (mononuclear); Lifestyles; Morbidity; Mutation; Oral cancer; p53 Protein; Patients; Peripheral blood mononuclear cells; Polymerase chain reaction; Polymorphism; Population; Restriction fragment length polymorphism; Risk factors; Senescence; Stomach cancer; Studies; Transcription; Tumor suppressor genes; Tumors
• ISSN: 2049-9434; 2049-9442
• DOI: 10.3892/br.2018.1070

Gastric cancer has the fourth highest morbidity rate of all cancers worldwide. Genetic factors including alterations in oncogenes and tumor suppressor genes serve an important role in gastric cancer development and progression. The gene acts as a tumor suppressor gene by regulating the cell cycle, DNA transcription and repair, apoptosis, senescence and genome stability. In addition to somatic mutations in cancer development, germline polymorphisms are also involved in different malignancies. The polymorphism of at codon 72 (Arg72Pro) is established as a common variant that increases susceptibility to various cancers. The present case-control study was conducted to evaluate the possible association between this polymorphism and gastric cancer in the Iranian population. A total of 59 patients with gastric cancer and 59 healthy controls were enrolled in the present study. Genomic DNA was extracted from peripheral blood mononuclear cells and genotype analysis was performed using a polymerase chain reaction-based restriction fragment length polymorphism assay. Genotype frequencies did not differ significantly between the patients and controls (P=0.4); the frequencies of the three genotypes Arg/Arg, Arg/Pro and Pro/Pro in gastric cancer patients were 28.8, 49.2 and 22.0%, and in controls were 37.3, 49.2 and 13.6%. Additionally, there were no differences in genotype frequencies based on tumor location, histological differentiation or tumor stage. Based on these findings, it may be concluded that the codon 72 polymorphism does not contribute to gastric cancer susceptibility in Northern Iran.