Variable Membrane Protein A of Flavescence Dorée Phytoplasma Binds the Midgut Perimicrovillar Membrane of Euscelidius variegatus and Promotes Adhesion to Its Epithelial Cells

• Published in: Applied and environmental microbiology 2018-04, Vol.84 (8), p.e02487-17
• Main Authors: Arricau-Bouvery, Nathalie, Duret, Sybille, Dubrana, Marie-Pierre, Batailler, Brigitte, Desqué, Delphine, Béven, Laure, Danet, Jean-Luc, Monticone, Michela, Bosco, Domenico, Malembic-Maher, Sylvie, Foissac, Xavier
• Format: Article
• Language: English
• Subjects: Adhesins, Bacterial - genetics; Adhesins, Bacterial - metabolism; Animals; Bacterial Adhesion - physiology; Epithelial Cells; Gastrointestinal Tract - microbiology; Hemiptera - microbiology; Invertebrate Microbiology; Life Sciences; Membrane Proteins - genetics; Membrane Proteins - metabolism; Microbiology and Parasitology; Microorganisms, Genetically-Modified; Phytoplasma - genetics; Phytoplasma - physiology; Plant Diseases - microbiology; Salivary Glands - microbiology; Spotlight
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.02487-17

Phytoplasmas are uncultivated plant pathogens and cell wall-less bacteria and are transmitted from plant to plant by hemipteran insects. The phytoplasma's circulative propagative cycle in insects requires the crossing of the midgut and salivary glands, and primary adhesion to cells is an initial step toward the invasion process. The flavescence dorée (FD) phytoplasma possesses a set of variable membrane proteins (Vmps) exposed on its surface, and this pathogen is suspected to interact with insect cells. The results showed that VmpA is expressed by the flavescence dorée phytoplasma present in the midgut and salivary glands. Phytoplasmas cannot be cultivated at present, and no mutant can be produced to investigate the putative role of Vmps in the adhesion of phytoplasma to insect cells. To overcome this difficulty, we engineered the mutant G/6, which lacks the ScARP adhesins, for VmpA expression and used VmpA-coated fluorescent beads to determine if VmpA acts as an adhesin in adhesion assays and ingestion assays. VmpA specifically interacted with insect cells in culture and promoted the retention of VmpA-coated beads to the midgut of In this latest case, VmpA-coated fluorescent beads were localized and embedded in the perimicrovillar membrane of the insect midgut. Thus, VmpA functions as an adhesin that could be essential in the colonization of the insect by the FD phytoplasmas. Phytoplasmas infect a wide variety of plants, ranging from wild plants to cultivated species, and are transmitted by different leafhoppers, planthoppers, and psyllids. The specificity of the phytoplasma-insect vector interaction has a major impact on the phytoplasma plant host range. As entry into insect cells is an obligate process for phytoplasma transmission, the bacterial adhesion to insect cells is a key step. Thus, studying surface-exposed proteins of phytoplasma will help to identify the adhesins implicated in the specific recognition of insect vectors. In this study, it is shown that the membrane protein VmpA of the flavescence dorée (FD) phytoplasma acts as an adhesin that is able to interact with cells of , the experimental vector of the FD phytoplasma.