Nuclear import of Cdc13 limits chromosomal capping

• Published in: Nucleic acids research 2018-04, Vol.46 (6), p.2975-2989
• Main Authors: Mersaoui, Sofiane Y, Bonnell, Erin, Wellinger, Raymund J
• Format: Article
• Language: English
• Subjects: Active Transport, Cell Nucleus; Amino Acid Sequence; Binding Sites - genetics; Cell Nucleus - metabolism; DNA - chemistry; DNA - genetics; DNA - metabolism; Genome Integrity, Repair and; Karyopherins - genetics; Karyopherins - metabolism; Mutation; Protein Binding; Protein Multimerization; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Sequence Homology, Amino Acid; Telomere - genetics; Telomere - metabolism; Telomere-Binding Proteins - chemistry; Telomere-Binding Proteins - genetics; Telomere-Binding Proteins - metabolism
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/gky085

Abstract Cdc13 is an essential protein involved in telomere maintenance and chromosome capping. Individual domain analyses on Cdc13 suggest the presence of four distinct OB-fold domains and one recruitment domain. However, it remained unclear how these sub-domains function in the context of the whole protein in vivo. Here, we use individual single domain deletions to address their roles in telomere capping. We find that the OB2 domain contains a nuclear localization signal that is essential for nuclear import of Cdc13 and therefore is required for chromosome capping. The karyopherin Msn5 is important for nuclear localization, and retention of Cdc13 in the nucleus also requires its binding to telomeres. Moreover, Cdc13 homodimerization occurs even if the protein is not bound to DNA and is in the cytoplasm. Hence, Cdc13 abundance in the nucleus and, in consequence, its capping function is strongly affected by nucleo-cytoplasmic transport as well as nuclear retention by DNA binding.