Lipid Peroxidation and Its Role in the Expression of NLRP1a and NLRP3 Genes in Testicular Tissue of Male Rats: a Model of Spinal Cord Injury

The majority of male patients with spinal cord injury (SCI) suffer from infertility. Nucleotide-binding oligomerization domain-like receptors NOD-like receptors (NLRs) are a kind of receptors that corporate in the inflammasome complex. Recent studies have introduced the inflammasome as the responsib...

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Published in:Iranian biomedical journal 2018-05, Vol.22 (3), p.151-159
Main Authors: Bazrafkan, Mahshid, Nikmehr, Banafsheh, Shahverdi, Abdolhossein, Hosseini, Seyed Reza, Hassani, Fatemeh, Poorhassan, Mahnaz, Mokhtari, Tahmineh, Abolhassani, Farid, Choobineh, Hamid, Beyer, Cordian, Hassanzadeh, Gholamreza
Format: Article
Language:English
Subjects:
Animal tissues
Animals
Cytokines
Epididymis
Full Length
Gene expression
Infertility
Inflammasomes
Injuries
Lipid peroxidation
Malondialdehyde
Oligomerization
Peroxidation
Rats
Reactive oxygen species
Receptors
Rodents
Semen
Spinal cord injuries
Surgery
Testes
Thorax
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container_issue 3
container_start_page 151
container_title Iranian biomedical journal
container_volume 22
creator Bazrafkan, Mahshid
Nikmehr, Banafsheh
Shahverdi, Abdolhossein
Hosseini, Seyed Reza
Hassani, Fatemeh
Poorhassan, Mahnaz
Mokhtari, Tahmineh
Abolhassani, Farid
Choobineh, Hamid
Beyer, Cordian
Hassanzadeh, Gholamreza
description The majority of male patients with spinal cord injury (SCI) suffer from infertility. Nucleotide-binding oligomerization domain-like receptors NOD-like receptors (NLRs) are a kind of receptors that corporate in the inflammasome complex. Recent studies have introduced the inflammasome as the responsible agent for secreting cytokines in semen. Reactive oxygen species (ROS) is one of the elements that trigger inflammasome activation. Genital infections in SCI can lead to ROS generation. We investigated the relation between lipid peroxidation and inflammasome complex activity in testicular tissue of SCI rats. Adult male rats (n=20), weighting 200-250 g, were included and divided into four groups: three experimental groups, including SCI1, SCI3, and SCI7, i.e. the rats were subjected to SCI procedure and sacrificed after one, three, and seven days, respectively and a control group. We performed a moderate, midline spinal contusion injury at thoracic level 10. The animals were anesthetized, and testes were collected for measurement of gene expression by real-time PCR. Caudal parts of epididymis were collected for malondialdehyde (MDA) measurement. No NLRP1a mRNA over expression was seen in the testes of control and SCI groups. After seven days from SCI surgery, NLRP3 mRNA expression was significantly increased in SCI7 animals (P≤0.05). There was a significant difference in MDA level in SCI7 versus control group, as well as SCI1 and SCI3 animals (P≤0.05). NLRP3 overexpression occurs due to the increased ROS production in testicular tissue of SCI rats.
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Nucleotide-binding oligomerization domain-like receptors NOD-like receptors (NLRs) are a kind of receptors that corporate in the inflammasome complex. Recent studies have introduced the inflammasome as the responsible agent for secreting cytokines in semen. Reactive oxygen species (ROS) is one of the elements that trigger inflammasome activation. Genital infections in SCI can lead to ROS generation. We investigated the relation between lipid peroxidation and inflammasome complex activity in testicular tissue of SCI rats. Adult male rats (n=20), weighting 200-250 g, were included and divided into four groups: three experimental groups, including SCI1, SCI3, and SCI7, i.e. the rats were subjected to SCI procedure and sacrificed after one, three, and seven days, respectively and a control group. We performed a moderate, midline spinal contusion injury at thoracic level 10. The animals were anesthetized, and testes were collected for measurement of gene expression by real-time PCR. Caudal parts of epididymis were collected for malondialdehyde (MDA) measurement. No NLRP1a mRNA over expression was seen in the testes of control and SCI groups. After seven days from SCI surgery, NLRP3 mRNA expression was significantly increased in SCI7 animals (P≤0.05). There was a significant difference in MDA level in SCI7 versus control group, as well as SCI1 and SCI3 animals (P≤0.05). 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Nucleotide-binding oligomerization domain-like receptors NOD-like receptors (NLRs) are a kind of receptors that corporate in the inflammasome complex. Recent studies have introduced the inflammasome as the responsible agent for secreting cytokines in semen. Reactive oxygen species (ROS) is one of the elements that trigger inflammasome activation. Genital infections in SCI can lead to ROS generation. We investigated the relation between lipid peroxidation and inflammasome complex activity in testicular tissue of SCI rats. Adult male rats (n=20), weighting 200-250 g, were included and divided into four groups: three experimental groups, including SCI1, SCI3, and SCI7, i.e. the rats were subjected to SCI procedure and sacrificed after one, three, and seven days, respectively and a control group. We performed a moderate, midline spinal contusion injury at thoracic level 10. The animals were anesthetized, and testes were collected for measurement of gene expression by real-time PCR. 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ispartof Iranian biomedical journal, 2018-05, Vol.22 (3), p.151-159
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source Freely Accessible Journals; Open Access: PubMed Central
subjects Animal tissues
Animals
Cytokines
Epididymis
Full Length
Gene expression
Infertility
Inflammasomes
Injuries
Lipid peroxidation
Malondialdehyde
Oligomerization
Peroxidation
Rats
Reactive oxygen species
Receptors
Rodents
Semen
Spinal cord injuries
Surgery
Testes
Thorax
title Lipid Peroxidation and Its Role in the Expression of NLRP1a and NLRP3 Genes in Testicular Tissue of Male Rats: a Model of Spinal Cord Injury
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