An immunohistochemical study of metroplastic surgical specimens from patients with a septate uterus

• Published in: Reproductive medicine and biology 2011-12, Vol.10 (4), p.277-282
• Main Authors: Makino, Tsunehisa, Cai, Li-yi, Izumi, Shun-ichiro, Yasuda, Masanori
• Format: Article
• Language: English
• Subjects: Blood flow; Blood vessels; CD34 antigen; Cervical carcinoma; Cervix; Congenital uterine anomaly; Endothelial cells; Gynecology; Hysterectomy; Image processing; Immunohistochemistry; Medicine; Medicine & Public Health; Metroplasty; Morphology; Myometrium; Patients; Pregnancy; Recurrent pregnancy loss; Reproductive Medicine; Urology; Uterine vessel distribution; Uterus
• ISSN: 1445-5781; 1447-0578
• DOI: 10.1007/s12522-011-0103-8

Purpose To elucidate the etiology of recurrent pregnancy loss in patients with congenital uterine anomalies, an immunohistochemical technique was used to quantitatively evaluate the vascular arrangement of septate uteri with respect to vascular density and morphology. Methods Nine specimens obtained from patients who had undergone metroplastic surgery for the treatment of a septate uterus and 10 control specimens from patients who had undergone a hysterectomy because of cervical carcinoma were used in this study. Formalin-fixed paraffin-embedded uterine specimens were then immunostained for CD34, which is specifically expressed in vascular endothelial cells. Results The mean blood vessel count (mean ± SD) for the myometrium was 149.7 ± 22.7/field in the septate uteri and 162.2 ± 36.4/field in the control uteri; these values were not significantly different. However, the total vessel cross-sectional areas, as evaluated quantitatively using the KS400 image analysis system, were 10350.4 ± 1024.3 μm 2 /field for the septate uteri and 12002.9 ± 2232.3 μm 2 /field for the control uteri; these values were significantly different ( p