A Real-Time Near-Infrared Fluorescence Imaging Method for the Detection of Oral Cancers in Mice Using an Indocyanine Green–Labeled Podoplanin Antibody

• Published in: Technology in cancer research & treatment 2018-01, Vol.17, p.1533033818767936-1533033818767936
• Main Authors: Ito, Akihiro, Ohta, Mitsuhiko, Kato, Yukinari, Inada, Shunko, Kato, Toshio, Nakata, Susumu, Yatabe, Yasushi, Goto, Mitsuo, Kaneda, Norio, Kurita, Kenichi, Nakanishi, Hayao, Yoshida, Kenji
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal; Early Detection of Cancer - instrumentation; Early Detection of Cancer - methods; Heterografts; Humans; Indocyanine Green; Membrane Glycoproteins - analysis; Methods; Mice; Mice, Nude; Molecular Imaging - instrumentation; Molecular Imaging - methods; Mouth Neoplasms - diagnostic imaging; Optical Imaging - instrumentation; Optical Imaging - methods; Oral cancer; Original; Squamous cell carcinoma; Squamous Cell Carcinoma of Head and Neck - diagnostic imaging; Tumors; Xenografts
• ISSN: 1533-0346; 1533-0338
• DOI: 10.1177/1533033818767936

Podoplanin is distinctively overexpressed in oral squamous cell carcinoma than oral benign neoplasms and plays a crucial role in the pathogenesis and metastasis of oral squamous cell carcinoma but its diagnostic application is quite limited. Here, we report a new near-infrared fluorescence imaging method using an indocyanine green (ICG)–labeled anti-podoplanin antibody and a desktop/a handheld ICG detection device for the visualization of oral squamous cell carcinoma–xenografted tumors in nude mice. Both near-infrared imaging methods using a desktop (in vivo imaging system: IVIS) and a handheld device (photodynamic eye: PDE) successfully detected oral squamous cell carcinoma tumors in nude mice in a podoplanin expression–dependent manner with comparable sensitivity. Of these 2 devices, only near-infrared imaging methods using a handheld device visualized oral squamous cell carcinoma xenografts in mice in real time. Furthermore, near-infrared imaging methods using the handheld device (PDE) could detect smaller podoplanin-positive oral squamous cell carcinoma tumors than a non-near-infrared, autofluorescence-based imaging method. Based on these results, a near-infrared imaging method using an ICG-labeled anti-podoplanin antibody and a handheld detection device (PDE) allows the sensitive, semiquantitative, and real-time imaging of oral squamous cell carcinoma tumors and therefore represents a useful tool for the detection and subsequent monitoring of malignant oral neoplasms in both preclinical and some clinical settings.