Postnatal development of lymphatic vasculature in the brain meninges

Background: Traditionally, the central nervous system (CNS) has been viewed as an immune‐privileged environment with no lymphatic vessels. This view was partially overturned by the discovery of lymphatic vessels in the dural membrane that surrounds the brain, in contact with the interior surface of...

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Published in:Developmental dynamics 2018-05, Vol.247 (5), p.741-753
Main Authors: Izen, Rebecca M., Yamazaki, Tomoko, Nishinaka‐Arai, Yoko, Hong, Young‐Kwon, Mukouyama, Yoh‐Suke
Format: Article
Language:English
Subjects:
Animals
Antibodies
Bacterial artificial chromosomes
Blood vessels
Brain
Brain - blood supply
Central nervous system
dura mater
Embryos
Lymphatic system
lymphatic vessels
Lymphatic Vessels - embryology
meningeal lymphatics
Meninges
Meninges - embryology
Mice
Mice, Transgenic
Neuroimaging
Olfactory bulb
Prox1
Sinuses
Skin
Skin - embryology
Skull
Superior sagittal sinus
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Summary:Background: Traditionally, the central nervous system (CNS) has been viewed as an immune‐privileged environment with no lymphatic vessels. This view was partially overturned by the discovery of lymphatic vessels in the dural membrane that surrounds the brain, in contact with the interior surface of the skull. We here examine the distribution and developmental timing of these lymphatic vessels. Results: Using the Prox1‐GFP BAC transgenic reporter and immunostaining with antibodies to lymphatic markers LYVE‐1, Prox1, and Podoplanin, we have carried out whole‐mount imaging of dural lymphatic vasculature at postnatal stages. We have found that between birth and postnatal day (P) 13, lymphatic vessels extend alongside dural blood vessels from the side of the skull toward the midline. Between P13 and P20, lymphatic vessels along the transverse sinuses reach the superior sagittal sinus (SSS) and extend along the SSS toward the olfactory bulb. Conclusions: Compared with the embryonic developmental timing of lymphatic vessels in other tissues, e.g. skin, dural lymphatic vessel development is dramatically delayed. This study provides useful anatomical data for continuing investigations of the fundamental mechanisms that underlie dural lymphatic vessel development. Developmental Dynamics 247:741–753, 2018. © 2018 Wiley Periodicals, Inc. Key Findings Lymphatic vessels, Meningeal lymphatics, Prox1, Dura mater.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.24624