Development of Nanoemulsions to Enhance the Antileishmanial Activity of Copaifera paupera Oleoresins

Based on the ethnopharmacological evidences about the antileishmanial activity of Copaifera spp. oleoresins, the effects of crude extracts and fractions of oleoresin of two specimens from Copaifera paupera were evaluated on Leishmania amazonensis and Leishmania infantum strains. The oleoresin rich i...

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Bibliographic Details
Published in:BioMed research international 2018-01, Vol.2018 (2018), p.1-9
Main Authors: Amaral, Ana Cláudia Fernandes, Basso, Silvia L., Ferreira, José Luiz P., Falcão, Deborah Q., Ramos, Aline de S., Rodrigues, Igor A., Silva, Jefferson Rocha de A.
Format: Article
Language:English
Subjects:
Acids
Antiprotozoal Agents - pharmacology
Copaifera
Copaifera multijuga
Copaifera officinalis
Emulsions
Emulsions - chemistry
Fabaceae - chemistry
Gas Chromatography-Mass Spectrometry
Leishmania amazonensis
Leishmania infantum - drug effects
Leishmania mexicana - drug effects
Leishmania tropica
Nanoemulsions
Nanoparticles
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Nanotechnology
Oils & fats
Oleoresins
Parasitic diseases
Particle Size
Pentamidine isethionate
Pharmaceutical sciences
Plant Extracts - pharmacology
Promastigotes
Sesquiterpenes - pharmacology
Surfactants
Tropical diseases
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Summary:Based on the ethnopharmacological evidences about the antileishmanial activity of Copaifera spp. oleoresins, the effects of crude extracts and fractions of oleoresin of two specimens from Copaifera paupera were evaluated on Leishmania amazonensis and Leishmania infantum strains. The oleoresin rich in α-copaene (38.8%) exhibited the best activity against L. amazonensis (IC50 = 62.5 μg/mL) and against L. infantum (IC50 = 65.9 μg/mL). The sesquiterpene α-copaene isolated was tested alone and exhibited high antileishmanial activity in vitro with IC50 values for L. amazonensis and L. infantum of 17.2 and 11.4 μg/mL, respectively. In order to increase antileishmanial activity, nanoemulsions containing copaiba oleoresin and α-copaene were developed and assayed against L. amazonensis and L. infantum promastigotes. The nanoemulsion containing α-copaene (NANOCOPAEN) showed the best activity against both species, with IC50 of 2.5 and 2.2 μg/mL, respectively. This is the first report about the antileishmanial activity of α-copaene.
ISSN:2314-6133
2314-6141
DOI:10.1155/2018/9781724