Directed reprogramming of comprehensively characterized dental pulp stem cells extracted from natal tooth

The aim of this study was to extensively characterise natal dental pulp stem cells (nDPSC) and assess their efficiency to generate human induced pluripotent stem cells (hiPSC). A number of distinguishing features prompted us to choose nDPSC over normal adult DPSC, in that they differed in cell surfa...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2018-04, Vol.8 (1), p.6168-13, Article 6168
Main Authors: Pisal, Rishikaysh V., Suchanek, Jakub, Siller, Richard, Soukup, Tomas, Hrebikova, Hana, Bezrouk, Ales, Kunke, David, Micuda, Stanislav, Filip, Stanislav, Sullivan, Gareth, Mokry, Jaroslav
Format: Article
Language:English
Subjects:
13
13/100
13/106
13/31
14
14/1
14/63
38
38/39
631/532/2435
c-Myc protein
Cell surface
Dental pulp
Efficiency
Embryo cells
Embryos
Fibroblasts
Gene expression
Humanities and Social Sciences
KLF4 protein
multidisciplinary
Myc protein
Oct-4 protein
Pluripotency
Science
Science (multidisciplinary)
Skin
Stem cells
Surface markers
Teeth
Transcription factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this study was to extensively characterise natal dental pulp stem cells (nDPSC) and assess their efficiency to generate human induced pluripotent stem cells (hiPSC). A number of distinguishing features prompted us to choose nDPSC over normal adult DPSC, in that they differed in cell surface marker expression and initial doubling time. In addition, nDPSC expressed 17 out of 52 pluripotency genes we analysed, and the level of expression was comparable to human embryonic stem cells (hESC). Ours is the first group to report comprehensive characterization of nDPSC followed by directed reprogramming to a pluripotent stem cell state. nDPSC yielded hiPSC colonies upon transduction with Sendai virus expressing the pluripotency transcription factors POU5F1, SOX2, c-MYC and KLF4 . nDPSC had higher reprogramming efficiency compared to human fibroblasts. nDPSC derived hiPSCs closely resembled hESC in terms of their morphology, expression of pluripotency markers and gene expression profiles. Furthermore, nDPSC derived hiPSCs differentiated into the three germ layers when cultured as embryoid bodies (EB) and by directed differentiation. Based on our findings, nDPSC present a unique marker expression profile compared with adult DPSC and possess higher reprogramming efficiency as compared with dermal fibroblasts thus proving to be more amenable for reprogramming.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-24421-z