Changes in gene expression in chronic allergy mouse model exposed to natural environmental PM2.5-rich ambient air pollution

• Published in: Scientific reports 2018-04, Vol.8 (1), p.6326-10, Article 6326
• Main Authors: Ouyang, Yuhui, Xu, Zhaojun, Fan, Erzhong, Li, Ying, Miyake, Kunio, Xu, Xianyan, Zhang, Luo
• Format: Article
• Language: English
• Subjects: 692/308/1426; 692/699/1785; A549 Cells; Air Pollutants - analysis; Air pollution; Air Pollution - analysis; Allergic diseases; Allergies; Animals; Environmental Exposure - analysis; Environmental Monitoring - methods; Eosinophils - metabolism; Female; Gene expression; HEK293 Cells; Humanities and Social Sciences; Humans; Hypersensitivity - genetics; Hypersensitivity - metabolism; Immune system; Innate immunity; Leukocytes (eosinophilic); Mice; Mice, Inbred BALB C; Mucosa; multidisciplinary; Nasal Mucosa - metabolism; NF-kappa B - metabolism; NF-κB protein; Nod1 protein; Nod1 Signaling Adaptor Protein - genetics; Nod1 Signaling Adaptor Protein - metabolism; Oligomerization; Particulate matter; Particulate Matter - adverse effects; Particulate Matter - analysis; Pollution; Science; Science (multidisciplinary); Signal transduction; Transcriptome - genetics; Triggering Receptor Expressed on Myeloid Cells-1 - genetics; Triggering Receptor Expressed on Myeloid Cells-1 - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-24831-z

Particulate matter (PM) air pollution has been associated with an increase in the incidence of chronic allergic diseases; however, the mechanisms underlying the effect of exposure to natural ambient air pollution in chronic allergic diseases have not been fully elucidated. In the present study, we aimed to investigate the cellular responses induced by exposure to natural ambient air pollution, employing a mouse model of chronic allergy. The results indicated that exposure to ambient air pollution significantly increased the number of eosinophils in the nasal mucosa. The modulation of gene expression profile identified a set of regulated genes, and the Triggering Receptor Expressed on Myeloid cells1(TREM1) signaling canonical pathway was increased after exposure to ambient air pollution. In vitro , PM2.5 increased Nucleotide-binding oligomerization domain-containing protein 1 (Nod1) and nuclear factor (NF)-κB signaling pathway activation in A549 and HEK293 cell cultures. These results suggest a novel mechanism by which, PM2.5 in ambient air pollution may stimulate the innate immune system through the PM2.5-Nod1-NF-κB axis in chronic allergic disease.