Two-Stage Isothermal Enzymatic Amplification for Concurrent Multiplex Molecular Detection

The wide array of pathogens responsible for infectious diseases makes it difficult to identify causative pathogens with single-plex tests. Although multiplex PCR detects multiple targets, it is restricted to centralized laboratories, which delays test results or makes multiplexing unavailable, depri...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2017-03, Vol.63 (3), p.714-722
Main Authors: Song, Jinzhao, Liu, Changchun, Mauk, Michael G, Rankin, Shelley C, Lok, James B, Greenberg, Robert M, Bau, Haim H
Format: Article
Language:English
Subjects:
Assaying
Colorimetry
Deoxyribonucleic acid
DNA
Drug resistance
Dyes
Experiments
Fluorescence
Fluorescent indicators
Format
Health care
Humans
Infections
Infectious diseases
Laboratories
Methods
Microfluidic Analytical Techniques
Microfluidics
Multiplex Polymerase Chain Reaction
Multiplexing
Nucleic Acid Amplification Techniques
Pathogens
Point-of-Care Systems
Polymerase chain reaction
Primers
Reactors
Recombinase
Smartphones
Target detection
Temperature
Vector-borne diseases
Viruses
Zika virus
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Summary:The wide array of pathogens responsible for infectious diseases makes it difficult to identify causative pathogens with single-plex tests. Although multiplex PCR detects multiple targets, it is restricted to centralized laboratories, which delays test results or makes multiplexing unavailable, depriving healthcare providers of critical, real-time information. To address the need for point-of-care (POC) highly multiplexed tests, we propose the 2-stage, nested-like, rapid (
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2016.263665