Detection of mRNAs of Carcinoembryonic Antigen and Nonspecific Cross‐reacting Antigen Genes in Colorectal Adenomas and Carcinomas by in situ Hybridization

Expression of mRNAs of carcinoembryonic antigen (CEA) and nonspecific cross‐reacting antigen (NCA) genes in colorectal carcinomas and adenomas was investigated by in situ hybridization (ISH) with specific biotinylated probes. Hybridization was clearly detected throughout the cytoplasm of 7 out of 15...

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Bibliographic Details
Published in:Cancer science 1990-11, Vol.81 (11), p.1149-1154
Main Authors: Higashide, Toshiyuki, Hinoda, Yuji, Itoh, Jun, Takahashi, Hiroki, Satoh, Yukihiko, Ibayashi, Yumiko, Imai, Kohzoh, Yachi, Akira
Format: Article
Language:English
Subjects:
Adenoma - genetics
Antigens
Antigens, Neoplasm - biosynthesis
Biological and medical sciences
Blotting, Northern
Carcinoembryonic antigen
Carcinoembryonic Antigen - genetics
Carcinoma - genetics
Cell Adhesion Molecules
Cells, Cultured
Colorectal carcinoma
Colorectal Neoplasms - genetics
Cytoplasm
DNA Probes
Gastroenterology. Liver. Pancreas. Abdomen
Gene Expression
Glycoproteins - biosynthesis
Humans
Hybrid Cells
Hybridization
In situ hybridization
In Vitro Techniques
Localization
Medical sciences
Nonspecific cross‐reacting antigen
Probes
RNA, Messenger - analysis
Tumors
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Summary:Expression of mRNAs of carcinoembryonic antigen (CEA) and nonspecific cross‐reacting antigen (NCA) genes in colorectal carcinomas and adenomas was investigated by in situ hybridization (ISH) with specific biotinylated probes. Hybridization was clearly detected throughout the cytoplasm of 7 out of 15 adenomas and 13 out of 15 carcinomas with the CEA cDNA probe, and in 6 out of 15 adenomas and 10 out of 15 carcinomas with the NCA cDNA probe. The intensity of signal appeared to be stronger in carcinomas than that in adenomas, and the CEA and NCA mRNAs were expressed together in most of the positive tissue specimens. On the other hand, noninvaded tissues adjacent to the carcinoma did not show any signal except for 4 cases faintly stained with the NCA probe. This finding was partly confirmed by Northern blot analysis which indicated that the specific bands for the CEA and NCA mRNAs were more intense in RNAs from carcinoma tissues than in those from adjacent noninvaded tissues. These data suggest that the ISH technique with biotinylated probes could be of use for analyzing expression and localization of CEA and related genes on tissue sections.
ISSN:0910-5050
1347-9032
1349-7006
1876-4673
DOI:10.1111/j.1349-7006.1990.tb02527.x