Purification and Characterization of Tumor Invasion‐inhibiting Factors

Tumor invasion‐inhibiting factors were purified from bovine liver using an in vitro system for estimating the tumor invasion ability. The acid‐ethanol extract of liver was subjected to ultrafiltration (Amicon PM10 membrane), and the fraction corresponding to the molecular weight range below 10,000 w...

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Bibliographic Details
Published in:Cancer science 1990-09, Vol.81 (9), p.909-914
Main Authors: Isoai, Atsushi, Giga‐Hama, Yuko, Shinkai, Kiyoko, Mukai, Mutsuko, Akedo, Hitoshi, Kumagai, Hiromichi
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids - analysis
Animals
Antineoplastic Agents - isolation & purification
Biological and medical sciences
Biological Factors - isolation & purification
Cattle
Chromatography, High Pressure Liquid
Ethanol
In Vitro Techniques
Invasion‐inhibiting factors
Liver
Liver - chemistry
Medical sciences
Metastasis
Molecular Weight
Neoplasm Metastasis
Neoplasms, Experimental - chemistry
Neoplasms, Experimental - pathology
Peptides
Peptides - isolation & purification
Purification
Rats
Tumor Cells, Cultured
Tumor invasion
Tumors
Ultrafiltration
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Summary:Tumor invasion‐inhibiting factors were purified from bovine liver using an in vitro system for estimating the tumor invasion ability. The acid‐ethanol extract of liver was subjected to ultrafiltration (Amicon PM10 membrane), and the fraction corresponding to the molecular weight range below 10,000 was further fractionated by ion‐exchange (DEAE‐Toyopearl), gel filtration (Bio‐Gel P6), and reverse‐phase (C18) chromatographies. Two types of active polypeptides with molecular weights of about 5,000 and 2,000 were purified and named IIF‐1 and IIF‐2, respectively. Both peptides inhibited tumor invasion with half‐maximum concentrations of 2–6 ng/ml in vitro. The amino acid compositions of both peptides were determined.
ISSN:0910-5050
1347-9032
1349-7006
1876-4673
DOI:10.1111/j.1349-7006.1990.tb02666.x