Establishment and Characterization of an Epithelial Cell Line with Quasi‐normal Chromosomes from a Tubular Adenoma of a Familial Polyposis Coli Patient

An epithelial cell line designated FPCK‐1 has been established from a tubular adenoma developing in a male familial polyposis coli (FPC) patient. The FPCK‐1 cells grow very slowly with adundant mucus production and have been maintained stably for 3 years in culture. No growth was evident either in s...

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Bibliographic Details
Published in:Cancer science 1991-02, Vol.82 (2), p.138-141
Main Authors: Kawaguchi, Tokuichi, Miyaki, Michiko, Masui, Tohru, Watanabe, Motoko, Ohta, Hirotoshi, Maruyama, Masakazu, Utakoji, Tadashi, Kitagawa, Tomoyuki
Format: Article
Language:English
Subjects:
Adenoma
Adenoma - genetics
Adenomatous Polyposis Coli - genetics
Adult
Biological and medical sciences
Carcinogenesis
Cell culture
Cell Line
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 22
Chromosomes, Human, Pair 5
Colon
Colon - pathology
Colon carcinogenesis
Epithelial cell line
Epithelial cells
Familial polyposis coli
Gastroenterology. Liver. Pancreas. Abdomen
Gene amplification
Genes, ras
Humans
In Vitro Techniques
Karyotypes
Male
Medical sciences
Multistep carcinogenesis
Myc protein
Point mutation
Polyposis
Polyposis coli
Rapid Communication
Ras protein
Tumor Cells, Cultured
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Summary:An epithelial cell line designated FPCK‐1 has been established from a tubular adenoma developing in a male familial polyposis coli (FPC) patient. The FPCK‐1 cells grow very slowly with adundant mucus production and have been maintained stably for 3 years in culture. No growth was evident either in soft agar or nude mice, FPCK‐1 cells present a normal male karyotype and do not show loss of specific loci on chromosomes 5,17,18, and 22 which have been reported to be lost frequently in human colon carcinomas. The cells have neither a point mutation on codon 12 of K‐ras gene nor gene amplification of myc, c‐H‐ras, and/or c‐K‐ras genes. These results thus suggest the existence of hitherto unknown causative event(s) underlying adenoma development in FPC patients. The FPCK‐1 cell line should prove useful for further analytical investigation of the multiple steps involved in human colon carcinogenesis.
ISSN:0910-5050
1347-9032
1349-7006
1876-4673
DOI:10.1111/j.1349-7006.1991.tb01820.x