Low Frequency of Rearrangements of the ret and trk Proto‐oncogenes in Japanese Thyroid Papillary Carcinomas

We investigated the frequency of rearrangements of the ret and trk proto‐oncogenes in Japanese thyroid tumors. DNAs from 38 thyroid papillary carcinomas and 14 follicular adenomas were analyzed by Southern blotting. Rearrangements of the ret and trk proto‐oncogenes were detected in one and two papil...

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Bibliographic Details
Published in:Cancer science 1992-07, Vol.83 (7), p.671-675
Main Authors: Wajjwalku, Worawidh, Nakamura, Shigeo, Hasegawa, Yasuhisa, Miyazaki, Koichi, Satoh, Yasuyuki, Funahashi, Hiroomi, Matsuyama, Mutsushi, Takahashi, Masahide
Format: Article
Language:English
Subjects:
Adenoma - genetics
Biological and medical sciences
Blotting, Northern
Carcinoma, Papillary - genetics
DNA, Neoplasm - genetics
Drosophila Proteins
Endocrinopathies
Gene Expression Regulation, Neoplastic - genetics
Gene Rearrangement - genetics
Goiter
Humans
Key words
Malignant tumors
Medical sciences
Oncogenes
Poly A - isolation & purification
Polymerase Chain Reaction
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-ret
Proto-Oncogenes - genetics
Rapid Communication
Rearrangement
Receptor Protein-Tyrosine Kinases
Receptor, trkA
ret and trk proto‐oncogenes
Ret protein
RNA - isolation & purification
RNA, Messenger
RNA-directed DNA polymerase
Southern blotting
Thyroid
Thyroid gland
Thyroid Neoplasms - genetics
Thyroid papillary carcinoma
Thyroid. Thyroid axis (diseases)
Transcription
Tumors
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Summary:We investigated the frequency of rearrangements of the ret and trk proto‐oncogenes in Japanese thyroid tumors. DNAs from 38 thyroid papillary carcinomas and 14 follicular adenomas were analyzed by Southern blotting. Rearrangements of the ret and trk proto‐oncogenes were detected in one and two papillary carcinomas, respectively, but not in follicular adenomas. Analysis by a reverse transcriptase‐polymerase chain reaction method showed that the ret rearrangement‐positive tumor contained the PTC/refTPCchimeric transcript, which was reported to be found specifically in thyroid tumors and adenomatous goiter. We also found that rearranged mRNA of the trk proto‐oncogene was expressed at high levels in one of two trk rearrangement‐positive tumors. Our results indicated that the frequency of rearrangements of these proto‐oncogenes in Japanese papillary carcinomas was much lower than that in Italian patients.
ISSN:0910-5050
1347-9032
1349-7006
1876-4673
DOI:10.1111/j.1349-7006.1992.tb01963.x