Differential Interaction of Platelet-Derived Extracellular Vesicles with Leukocyte Subsets in Human Whole Blood

Secretion and exchange of biomolecules via extracellular vesicles (EVs) are crucial mechanisms in intercellular communication, and the roles of EVs in infection, inflammation, or thrombosis have been increasingly recognized. EVs have emerged as central players in immune regulation and can enhance or...

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Bibliographic Details
Published in:Scientific reports 2018-04, Vol.8 (1), p.6598-11, Article 6598
Main Authors: Weiss, René, Gröger, Marion, Rauscher, Sabine, Fendl, Birgit, Eichhorn, Tanja, Fischer, Michael B., Spittler, Andreas, Weber, Viktoria
Format: Article
Language:English
Subjects:
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Blood
Blood platelets
CD14 antigen
CD16 antigen
Cell signaling
Confocal microscopy
Extracellular vesicles
Flow cytometry
Granulocytes
Humanities and Social Sciences
Immune response
Immunoregulation
Leukocytes (granulocytic)
Lymphocytes
Lymphocytes B
Lymphocytes T
Monocytes
multidisciplinary
Platelets
Science
Science (multidisciplinary)
Subpopulations
Thromboembolism
Thrombosis
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Summary:Secretion and exchange of biomolecules via extracellular vesicles (EVs) are crucial mechanisms in intercellular communication, and the roles of EVs in infection, inflammation, or thrombosis have been increasingly recognized. EVs have emerged as central players in immune regulation and can enhance or suppress the immune response, depending on the state of donor and recipient cells. We investigated the interaction of blood cell-derived EVs with leukocyte subpopulations (monocytes and their subsets, granulocytes, B cells, T cells, and NK cells) directly in whole blood using a combination of flow cytometry, imaging flow cytometry, cell sorting, and high resolution confocal microscopy. Platelet-derived EVs constituted the majority of circulating EVs and were preferentially associated with granulocytes and monocytes, while they scarcely interacted with lymphocytes. Further flow cytometric differentiation of monocyte subsets provided clear indications for a preferential association of platelet-derived EVs with intermediate (CD14 ++ CD16 + ) monocytes in whole blood.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-25047-x