Isolation of STLV–I from Orangutan, a Great Ape Species in Southeast Asia, and Its Relation to Other HTLV–Is/STLV–Is

To study the evolutionary origin of human T–lymphotropic virus type I/simian T–lymphotropic virus type I (HTLV–I/STLV–I), we isolated and characterized STLV–I from orangutans (Pongo pygmaeus). Plasma samples from 3 out of 41 animals examined were reactive by particle agglutination and immunofluoresc...

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Bibliographic Details
Published in:Cancer science 1997-01, Vol.88 (1), p.1-4
Main Authors: Ibuki, Kentaro, Ido, Eiji, Setiyaningsih, Surachmi, Yamashita, Masahiro, Agus, Lelana R. P., Takehisa, Jun, Miura, Tomoyuki, Dondin, Sajuthi, Hayami, Masanori
Format: Article
Language:English
Subjects:
Agglutination
AIDS/HIV
Animals
Base Sequence
Biological and medical sciences
Divergence
Fundamental and applied biological sciences. Psychology
Human T-lymphotropic virus 1 - classification
Human T-lymphotropic virus 1 - genetics
Immunofluorescence
Indonesia
Leukocytes (mononuclear)
Long terminal repeat
LTR
Microbiology
Molecular Sequence Data
Monkeys & apes
Orangutan
Peripheral blood mononuclear cells
Phylogenetic analysis
Phylogeny
Polymerase chain reaction
Pongo pygmaeus
Pongo pygmaeus - virology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
simian T-lymphotropic virus
Simian T-lymphotropic virus 1 - classification
Simian T-lymphotropic virus 1 - genetics
Simian T-lymphotropic virus 1 - isolation & purification
STLV–I/HTLV
Virology
Western blotting
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Summary:To study the evolutionary origin of human T–lymphotropic virus type I/simian T–lymphotropic virus type I (HTLV–I/STLV–I), we isolated and characterized STLV–I from orangutans (Pongo pygmaeus). Plasma samples from 3 out of 41 animals examined were reactive by particle agglutination and immunofluorescence, and one of these three was confirmed to be anti–HTLV–I antibody–positive by western blotting (WB). Cultured peripheral blood mononuclear cells from the WB–positive orangutan were reactive to anti–STLV–I–positive rhesus monkey plasma. The proviral long terminal repeat region was amplified by polymerase chain reaction and sequenced. A phylogenetic analysis indicated that orangutan STLV–I is related to the Melanesian group of HTLV–Is and other Asian STLV–Is, but the degree of divergence is considerable.
ISSN:0910-5050
1347-9032
1349-7006
1876-4673
DOI:10.1111/j.1349-7006.1997.tb00293.x