Visualizing the Interior Architecture of Focal Adhesions with High-Resolution Traction Maps

Focal adhesions (FAs) are micron-sized protein assemblies that coordinate cell adhesion, migration, and mechanotransduction. How the many proteins within FAs are organized into force sensing and transmitting structures is poorly understood. We combined fluorescent molecular tension sensors with supe...

Full description

Saved in:
Bibliographic Details
Published in:Nano letters 2015-04, Vol.15 (4), p.2220-2228
Main Authors: Morimatsu, Masatoshi, Mekhdjian, Armen H, Chang, Alice C, Tan, Steven J, Dunn, Alexander R
Format: Article
Language:English
Subjects:
Adhesion
Adhesiveness
Animals
Cells, Cultured
Cytoskeletal Proteins - metabolism
Fluorescence Resonance Energy Transfer - methods
Focal Adhesions - physiology
Humans
Image Enhancement - methods
Mechanotransduction, Cellular - physiology
Membrane Proteins - metabolism
Mice
Microscopy
Microscopy, Fluorescence - methods
Molecular Imaging - methods
Molecular structure
Nanostructure
Proteins
Sensors
Stress, Mechanical
Tensile Strength - physiology
Traction
Transducers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Focal adhesions (FAs) are micron-sized protein assemblies that coordinate cell adhesion, migration, and mechanotransduction. How the many proteins within FAs are organized into force sensing and transmitting structures is poorly understood. We combined fluorescent molecular tension sensors with super-resolution light microscopy to visualize traction forces within FAs with
ISSN:1530-6984
1530-6992
DOI:10.1021/nl5047335