Adaptation of Amoeba Plate Test To Recover Legionella Strains from Clinical Samples

The isolation of from respiratory samples is the gold standard for diagnosis of Legionnaires' disease (LD) and enables epidemiological studies and outbreak investigations. The purpose of this work was to adapt and to evaluate the performance of an amoebic coculture procedure (the amoeba plate t...

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Bibliographic Details
Published in:Journal of clinical microbiology 2018-05, Vol.56 (5), p.e01361-17
Main Authors: Descours, G, Hannetel, H, Reynaud, J V, Ranc, A G, Beraud, L, Kolenda, C, Campese, C, Lina, G, Ginevra, C, Jarraud, S
Format: Article
Language:English
Subjects:
Bacteriology
Bioengineering
Human health and pathology
Infectious diseases
Life Sciences
Microbiology and Parasitology
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Summary:The isolation of from respiratory samples is the gold standard for diagnosis of Legionnaires' disease (LD) and enables epidemiological studies and outbreak investigations. The purpose of this work was to adapt and to evaluate the performance of an amoebic coculture procedure (the amoeba plate test [APT]) for the recovery of strains from respiratory samples, in comparison with axenic culture and liquid-based amoebic coculture (LAC). Axenic culture, LAC, and APT were prospectively performed with 133 respiratory samples from patients with LD. The sensitivities and times to results for the three techniques were compared. Using the three techniques, strains were isolated in 46.6% ( = 62) of the 133 respiratory samples. The sensitivity of axenic culture was 42.9% ( = 57), that of LAC was 30.1% ( = 40), and that of APT was 36.1% ( = 48). Seven samples were positive by axenic culture only; for those samples, there were
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01361-17