β‐Catenin Accumulation and Mutation of Exon 3 of the β‐Catenin Gene in Hepatocellular Carcinoma

study was conducted to clarify the contribution of β‐catenin accumulation and mutation of the β‐catenin gene to hepatocarcinogenesis. β‐Catenin accumulation was examined immunohistochemically in 38 paired samples of hepatocellular carcinoma (HCC) and corresponding non‐cancerous liver tissue. Gene mu...

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Bibliographic Details
Published in:Cancer science 1999-12, Vol.90 (12), p.1301-1309
Main Authors: Kondo, Yutaka, Kanai, Yae, Sakamoto, Michiie, Genda, Takuya, Mizokami, Masashi, Ueda, Ryuzo, Hirohashi, Setsuo
Format: Article
Language:English
Subjects:
Adult
Aged
beta Catenin
Biological and medical sciences
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - metabolism
Carcinoma, Hepatocellular - pathology
Catenin
Cell membranes
Cirrhosis
Conformation
Cytoplasm
Cytoskeletal Proteins - genetics
Cytoskeletal Proteins - metabolism
Exons - genetics
Female
Gastroenterology. Liver. Pancreas. Abdomen
Gene polymorphism
Hepatitis
Hepatocellular carcinoma
Humans
Liver cancer
Liver cirrhosis
Liver Neoplasms - genetics
Liver Neoplasms - metabolism
Liver Neoplasms - pathology
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Male
Medical sciences
Middle Aged
Mutation
Point mutation
Polymerase chain reaction
Portal vein
Single strand conformation polymorphism
Trans-Activators
Tumors
β‐Catenin
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Summary:study was conducted to clarify the contribution of β‐catenin accumulation and mutation of the β‐catenin gene to hepatocarcinogenesis. β‐Catenin accumulation was examined immunohistochemically in 38 paired samples of hepatocellular carcinoma (HCC) and corresponding non‐cancerous liver tissue. Gene mutation was analyzed by polymerase chain reaction‐single strand conformation polymorphism (PCR‐SSCP) and direct sequencing using intronic primers encompassing exon 3. Neither accumulation nor mutation was detected in non‐cancerous liver tissues that showed no remarkable histological features, chronic hepatitis or liver cirrhosis. Accumulation of β‐catenin was seen in the nucleus, cytoplasm or cell membrane in 15 of 38 (39%) HCC samples, and gene mutation was seen in 9 of 38 (24%) HCC samples. Although there was a significant correlation between accumulation and mutation (P
ISSN:0910-5050
1347-9032
1349-7006
1876-4673
DOI:10.1111/j.1349-7006.1999.tb00712.x