Silencing of the CD44 Gene by CpG Methylation in a Human Gastric Carcinoma Cell Line

We analyzed 8 human gastric carcinoma cell lines for the expression of CD44 by northern blot analysis and reverse transcription‐polymerase chain reaction (RT‐PCR), and identified 1 cell line MKN‐28 that did not express CD44. In an attempt to clarify the mechanism responsible for the inactivation of...

Full description

Saved in:
Bibliographic Details
Published in:Cancer science 1999-05, Vol.90 (5), p.485-489
Main Authors: Sato, Sunao, Yokozaki, Hiroshi, Yasui, Wataru, Nikai, Hiromasa, Tahara, Eiichi
Format: Article
Language:English
Subjects:
Azacytidine
Biological and medical sciences
CD44
CD44 antigen
CpG islands
Deoxyribonucleic acid
Dinucleoside Phosphates
Dissemination
DNA
DNA Methylation
Gastric cancer
Gastric carcinoma
Gene expression
Gene Expression Regulation, Neoplastic - physiology
Humans
Hyaluronan Receptors - genetics
Medical sciences
Methylation
Polymerase chain reaction
Promoter Regions, Genetic
Rapid Communication
Reverse Transcriptase Polymerase Chain Reaction
Reverse transcription
Stomach Neoplasms - genetics
Tumor cell
Tumor cell lines
Tumor Cells, Cultured
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We analyzed 8 human gastric carcinoma cell lines for the expression of CD44 by northern blot analysis and reverse transcription‐polymerase chain reaction (RT‐PCR), and identified 1 cell line MKN‐28 that did not express CD44. In an attempt to clarify the mechanism responsible for the inactivation of CD44 gene expression in this cell line, we investigated the methylation status around the promoter region of CD44 gene by digestion of the DNA with the methylation‐sensitive restriction enzyme Hpa II. The promoter region of CD44 in MKN‐28 revealed hypermethylation, whereas other CD44‐positive cell lines did not. Furthermore, treatment of MKN‐28 with the demethylating agent 5‐azacytidine restored the expression of the gene. These results suggest that CD44 expression is controlled by a DNA hypermethylation mechanism in MKN‐28.
ISSN:0910-5050
1347-9032
1349-7006
1876-4673
DOI:10.1111/j.1349-7006.1999.tb00773.x