Group I chitin deacetylases are essential for higher order organization of chitin fibers in beetle cuticle

• Published in: The Journal of biological chemistry 2018-05, Vol.293 (18), p.6985-6995
• Main Authors: Noh, Mi Young, Muthukrishnan, Subbaratnam, Kramer, Karl J., Arakane, Yasuyuki
• Format: Article
• Language: English
• Subjects: Alternative Splicing; Amidohydrolases - genetics; Amidohydrolases - metabolism; Animal Scales - metabolism; Animal Scales - ultrastructure; Animals; Chitin - metabolism; chitin deacetylase (CDA); double-stranded RNA (dsRNA); electron microscopy (EM); elytron; extracellular matrix; Gene Expression Regulation, Enzymologic; Glycobiology and Extracellular Matrices; immunohistochemistry; Insect Proteins - genetics; Insect Proteins - metabolism; Microscopy, Electron, Transmission; Molecular Docking Simulation; Phenotype; pore canal; RNA Interference; RNA interference (RNAi); Tribolium - enzymology; Tribolium castaneum; Wings, Animal - metabolism; Wings, Animal - ultrastructure
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.RA117.001454

Roles in the organization of the cuticle (exoskeleton) of two chitin deacetylases (CDAs) belonging to group I, TcCDA1 and TcCDA2, as well as two alternatively spliced forms of the latter, TcCDA2a and TcCDA2b, from the red flour beetle, Tribolium castaneum, were examined in different body parts using transmission EM and RNAi. Even though all TcCDAs are co-expressed in cuticle-forming cells from the hardened forewing (elytron) and ventral abdomen, as well as in the softer hindwing and dorsal abdomen, there are significant differences in the tissue specificity of expression of the alternatively spliced transcripts. Loss of either TcCDA1 or TcCDA2 protein by RNAi causes abnormalities in organization of chitinous horizontal laminae and vertical pore canals in all regions of the procuticle of both the hard and soft cuticles. Simultaneous RNAi for TcCDA1 and TcCDA2 produces the most serious abnormalities. RNAi of either TcCDA2a or TcCDA2b affects cuticle integrity to some extent. Following RNAi, there is accumulation of smaller disorganized fibers in both the horizontal laminae and pore canals, indicating that TcCDAs play a critical role in elongation/organization of smaller nanofibers into longer fibers, which is essential for structural integrity of both hard/thick and soft/thin cuticles. Immunolocalization of TcCDA1 and TcCDA2 proteins and effects of RNAi on their accumulation indicate that these two proteins function in concert exclusively in the assembly zone in a step involving the higher order organization of the procuticle.