Strong associations between plant genotypes and bacterial communities in a natural salt marsh

• Published in: Ecology and evolution 2018-05, Vol.8 (9), p.4721-4730
• Main Authors: Zogg, Gregory P., Travis, Steven E., Brazeau, Daniel A.
• Format: Article
• Language: English
• Subjects: Bacteria; Community composition; Flooding; Genetic diversity; Genetic markers; Genetics; Genotypes; growth form; Herbivores; Microbial activity; Microorganisms; Microsatellites; Original Research; plant genotypes; plant–soil (below‐ground) interactions; Rhizosphere; salt marsh; Salt marshes; Soil structure; Spartina alterniflora; Terrestrial ecosystems; Terrestrial environments; Wetlands
• ISSN: 2045-7758; 2045-7758
• DOI: 10.1002/ece3.4105

Although microbial communities have been shown to vary among plant genotypes in a number of experiments in terrestrial ecosystems, relatively little is known about this relationship under natural conditions and outside of select model systems. We reasoned that a salt marsh ecosystem, which is characterized by twice‐daily flooding by tides, would serve as a particularly conservative test of the strength of plant–microbial associations, given the high degree of abiotic regulation of microbial community assembly resulting from alternating periods of inundation and exposure. Within a salt marsh in the northeastern United States, we characterized genotypes of the foundational plant Spartina alterniflora using microsatellite markers, and bacterial metagenomes within marsh soil based on pyrosequencing. We found significant differences in bacterial community composition and diversity between bulk and rhizosphere soil, and that the structure of rhizosphere communities varied depending on the growth form of, and genetic variation within, the foundational plant S. alterniflora. Our results indicate that there are strong plant–microbial associations within a natural salt marsh, thereby contributing to a growing body of evidence for a relationship between plant genotypes and microbial communities from terrestrial ecosystems and suggest that principles of community genetics apply to this wetland type. Although microbial communities have been shown to vary among plant genotypes in a number of experiments in terrestrial ecosystems, relatively little is known about this relationship under natural conditions and outside of select model systems. Within a salt marsh in the northeastern United States, we found evidence for a strong association between plants and microbes, including significant differences in bacterial community composition and diversity between bulk and rhizosphere soil, and that the structure of rhizosphere communities varied depending on the growth form of, and genetic variation within, the foundational plant Spartina alterniflora.