Exploring the Sea Urchin Neuropeptide Landscape by Mass Spectrometry

Neuropeptides are essential cell-to-cell signaling messengers and serve important regulatory roles in animals. Although remarkable progress has been made in peptide identification across the Metazoa, for some phyla such as Echinodermata, limited neuropeptides are known and even fewer have been verif...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2018-05, Vol.29 (5), p.923-934
Main Authors: Monroe, Eric B., Annangudi, Suresh P., Wadhams, Andinet A., Richmond, Timothy A., Yang, Ning, Southey, Bruce R., Romanova, Elena V., Schoofs, Liliane, Baggerman, Geert, Sweedler, Jonathan V.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Bioinformatics
Biotechnology
Chemistry
Chemistry and Materials Science
Focus: 29th Sanibel Conference
Homology
Invertebrates
Mass spectrometry
Nerves
Nervous system
Neuropeptides
Organic Chemistry
Peptides
Peptidomics
Bridging the Gap Between Proteomics and Metabolomics by MS: Research Article
Proteins
Proteomics
Scientific imaging
Signaling
Spectroscopy
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Summary:Neuropeptides are essential cell-to-cell signaling messengers and serve important regulatory roles in animals. Although remarkable progress has been made in peptide identification across the Metazoa, for some phyla such as Echinodermata, limited neuropeptides are known and even fewer have been verified on the protein level. We employed peptidomic approaches using bioinformatics and mass spectrometry (MS) to experimentally confirm 23 prohormones and to characterize a new prohormone in nervous system tissue from Strongylocentrotus purpuratus , the purple sea urchin. Ninety-three distinct peptides from known and novel prohormones were detected with MS from extracts of the radial nerves, many of which are reported or experimentally confirmed here for the first time, representing a large-scale study of neuropeptides from the phylum Echinodermata. Many of the identified peptides and their precursor proteins have low homology to known prohormones from other species/phyla and are unique to the sea urchin. By pairing bioinformatics with MS, the capacity to characterize novel peptides and annotate prohormone genes is enhanced. Graphical Abstract
ISSN:1044-0305
1879-1123
DOI:10.1007/s13361-018-1898-x