Long non-coding RNA AFAP1-antisense RNA 1 promotes the proliferation, migration and invasion of gastric cancer cells and is associated with poor patient survival

Gastric cancer (GC) is the second-leading cause of cancer-associated mortality worldwide. AFAP1-antisense RNA 1 (AFAP1-AS1), a long non-coding RNA (lncRNA), is believed to promote the aggressive progression of cancer; however, its role in GC remains largely unknown. In the present study, the express...

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Bibliographic Details
Published in:Oncology letters 2018-06, Vol.15 (6), p.8620-8626
Main Authors: Zhao, Huazhou, Zhang, Kecheng, Wang, Ting, Cui, Jianxin, Xi, Hongqing, Wang, Yi, Song, Yanjing, Zhao, Xudong, Wei, Bo, Chen, Lin
Format: Article
Language:English
Subjects:
Antisense RNA
Biotechnology
Care and treatment
Cell adhesion & migration
Cell culture
Cell growth
Development and progression
Gastric cancer
Gene expression
Genetic aspects
Health aspects
Infections
Medical prognosis
Metastasis
Mortality
Proteins
Stomach cancer
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Summary:Gastric cancer (GC) is the second-leading cause of cancer-associated mortality worldwide. AFAP1-antisense RNA 1 (AFAP1-AS1), a long non-coding RNA (lncRNA), is believed to promote the aggressive progression of cancer; however, its role in GC remains largely unknown. In the present study, the expression of AFAP1-AS1 in GC tissues and cell lines was measured using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Knockdown of AFAP1-AS1 was performed using a lentiviral vector containing a short hairpin RNA. The proliferation of GC cells was measured using Cell Counting kit-8. The migration and invasion of GC cells were analyzed using a QCM Laminin Migration Assay kit and a Cell Invasion Assay kit. The levels of epithelial-mesenchymal transition (EMT)-associated proteins were detected by western blot analysis. The cut-off value of the expression of AFAP1-AS1 was evaluated using receiver operating characteristic (ROC) curves and patient survival rate was analyzed using Kaplan-Meier. The expression of AFAP1-AS1 was significantly increased in the primary tumor tissues of GC patients with lymph node metastasis or tumor node metastasis stage (stage III or IV; P
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2018.8389