First Report of the Presence of Hepatitis E Virus in Scottish-Harvested Shellfish Purchased at Retail Level

Shellfish samples ( n  = 310) purchased from local supermarkets were analysed for the presence of hepatitis E virus (HEV) by nested RT-PCR and real-time qRT-PCR. Overall, 2.9% of samples tested positive for the presence of HEV. Phylogenetic analysis of HEV sequences revealed all as being genotype 3...

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Bibliographic Details
Published in:Food and environmental virology 2018-06, Vol.10 (2), p.217-221
Main Authors: O’Hara, Zoe, Crossan, Claire, Craft, John, Scobie, Linda
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Bivalvia - virology
Brief Communication
Chemistry/Food Science
Food Contamination - analysis
Food Microbiology
Food Science
Genotypes
Hepatitis
Hepatitis E - epidemiology
Hepatitis E - virology
Hepatitis E virus - genetics
Hepatitis E virus - isolation & purification
Humans
Phylogeny
Polymerase chain reaction
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - genetics
RNA, Viral - isolation & purification
Scotland - epidemiology
Shellfish
Shellfish - virology
Supermarkets
Virology
Viruses
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Summary:Shellfish samples ( n  = 310) purchased from local supermarkets were analysed for the presence of hepatitis E virus (HEV) by nested RT-PCR and real-time qRT-PCR. Overall, 2.9% of samples tested positive for the presence of HEV. Phylogenetic analysis of HEV sequences revealed all as being genotype 3 HEV. This is the first report of the detection of HEV in commercially sold shellfish in Scotland. These findings may encourage further research that will help address the gaps in the knowledge in respect to foodborne transmission of HEV in Scotland and the rest of the United Kingdom.
ISSN:1867-0334
1867-0342
DOI:10.1007/s12560-018-9337-5