Molecular detection of Hepatozoon canis in dogs from Kerala

India has a wide range of agro-climatic zones which is highly conducive for a diverse range of vectors and canines are continuously exposed to the risk of spectrum of tick borne protozoan diseases. The brown dog tick, Rhipicephalus sanguineus is widely prevalent among dogs in Kerala and there is a h...

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Bibliographic Details
Published in:Journal of parasitic diseases 2018-06, Vol.42 (2), p.287-290
Main Authors: Lakshmanan, Bindu, Jose, K. Jain, George, Arun, Usha, N. P., Devada, K.
Format: Article
Language:English
Subjects:
Arachnids
Dogs
Gelatin
Health Promotion and Disease Prevention
Hepatozoon canis
Infectious Diseases
Leukocytes (neutrophilic)
Medicine
Medicine & Public Health
Original
Original Article
Phylogeny
Protozoa
rRNA 18S
Vectors
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Summary:India has a wide range of agro-climatic zones which is highly conducive for a diverse range of vectors and canines are continuously exposed to the risk of spectrum of tick borne protozoan diseases. The brown dog tick, Rhipicephalus sanguineus is widely prevalent among dogs in Kerala and there is a high prevalence of this tick transmitted Babesia and Ehrlichia spp. infection. However, the incidence of Hepatozoon canis transmitted by the same tick species had not been reported in the state since 2004. Preliminary screening of client owned dogs revealed six dogs to be positive for typical gelatin capsule shaped gamonts of H. canis within neutrophils in blood smear by microscopic examination. A PCR assay was standardized to amplify a specific 737 bp fragment of 18S rRNA gene of H. canis. Phylogenetic analysis revealed closest relationship with West Indies isolate deposited at GenBank database. The present study records the molecular detection of this haemoparasite in the state, for the first time.
ISSN:0971-7196
0975-0703
DOI:10.1007/s12639-018-0998-7