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Using an environmentally-relevant panel of Gram-negative bacteria to assess the toxicity of polyallylamine hydrochloride-wrapped gold nanoparticles
We aim to establish the effect of environmental diversity in evaluating nanotoxicity to bacteria. We assessed the toxicity of 4 nm polyallylamine hydrochloride-wrapped gold nanoparticles (PAH AuNPs) to a panel of bacteria from diverse environmental niches. The bacteria experienced a range of toxicit...
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| Published in: | Environmental science. Nano 2018-02, Vol.5 (2), p.279-288 |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
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| cited_by | cdi_FETCH-LOGICAL-c469t-a7fdcd109a8b50c0ec775666ba8ed5c9233e367a2e1c7996246f2d1b4a903ba63 |
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| cites | cdi_FETCH-LOGICAL-c469t-a7fdcd109a8b50c0ec775666ba8ed5c9233e367a2e1c7996246f2d1b4a903ba63 |
| container_end_page | 288 |
| container_issue | 2 |
| container_start_page | 279 |
| container_title | Environmental science. Nano |
| container_volume | 5 |
| creator | Buchman, Joseph T Rahnamoun, Ali Landy, Kaitlin M Zhang, Xi Vartanian, Ariane M Jacob, Lisa M Murphy, Catherine J Hernandez, Rigoberto Haynes, Christy L |
| description | We aim to establish the effect of environmental diversity in evaluating nanotoxicity to bacteria. We assessed the toxicity of 4 nm polyallylamine hydrochloride-wrapped gold nanoparticles (PAH AuNPs) to a panel of bacteria from diverse environmental niches. The bacteria experienced a range of toxicities as evidenced by the different minimum bactericidal concentrations determined; the sensitivities of the bacteria was
A. vinelandii
=
P. aeruginosa
>
S. oneidensis
MR-4 >
A. baylyi
>
S. oneidensis
MR-1. Interactions between gold nanoparticles and molecular components of the cell wall were investigated by TEM, flow cytometry, and computational modeling. Binding results showed a general trend that bacteria with smooth lipopolysaccharides (LPS) bind more PAH AuNPs than bacteria with rough LPS. Computational models reveal that PAH migrates to phosphate groups in the core of the LPS structure. Overall, our results demonstrate that simple interactions between nanoparticles and the bacterial cell wall cannot fully account for observed trends in toxicity, which points to the importance of establishing more comprehensive approaches for modeling environmental nanotoxicity.
We aim to establish the effect of bacterial diversity in assessing nanotoxicity. |
| doi_str_mv | 10.1039/c7en00832e |
| format | article |
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A. vinelandii
=
P. aeruginosa
>
S. oneidensis
MR-4 >
A. baylyi
>
S. oneidensis
MR-1. Interactions between gold nanoparticles and molecular components of the cell wall were investigated by TEM, flow cytometry, and computational modeling. Binding results showed a general trend that bacteria with smooth lipopolysaccharides (LPS) bind more PAH AuNPs than bacteria with rough LPS. Computational models reveal that PAH migrates to phosphate groups in the core of the LPS structure. Overall, our results demonstrate that simple interactions between nanoparticles and the bacterial cell wall cannot fully account for observed trends in toxicity, which points to the importance of establishing more comprehensive approaches for modeling environmental nanotoxicity.
We aim to establish the effect of bacterial diversity in assessing nanotoxicity.</description><identifier>ISSN: 2051-8153</identifier><identifier>EISSN: 2051-8161</identifier><identifier>DOI: 10.1039/c7en00832e</identifier><identifier>PMID: 29805793</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Bacteria ; Computation ; Computer applications ; Cytometry ; Environment models ; Environmental effects ; Flow cytometry ; Gold ; Gram-negative bacteria ; Interactions ; Lipopolysaccharides ; Mathematical models ; Modelling ; Nanoparticles ; Niches ; Phosphates ; Polyallylamine ; Polyallylamine hydrochloride ; Toxicity</subject><ispartof>Environmental science. Nano, 2018-02, Vol.5 (2), p.279-288</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-a7fdcd109a8b50c0ec775666ba8ed5c9233e367a2e1c7996246f2d1b4a903ba63</citedby><cites>FETCH-LOGICAL-c469t-a7fdcd109a8b50c0ec775666ba8ed5c9233e367a2e1c7996246f2d1b4a903ba63</cites><orcidid>0000-0001-5827-8513 ; 0000-0001-7066-5575 ; 0000-0002-5420-5867 ; 0000-0002-8275-8469 ; 0000-0001-8526-7414</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29805793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Buchman, Joseph T</creatorcontrib><creatorcontrib>Rahnamoun, Ali</creatorcontrib><creatorcontrib>Landy, Kaitlin M</creatorcontrib><creatorcontrib>Zhang, Xi</creatorcontrib><creatorcontrib>Vartanian, Ariane M</creatorcontrib><creatorcontrib>Jacob, Lisa M</creatorcontrib><creatorcontrib>Murphy, Catherine J</creatorcontrib><creatorcontrib>Hernandez, Rigoberto</creatorcontrib><creatorcontrib>Haynes, Christy L</creatorcontrib><title>Using an environmentally-relevant panel of Gram-negative bacteria to assess the toxicity of polyallylamine hydrochloride-wrapped gold nanoparticles</title><title>Environmental science. Nano</title><addtitle>Environ Sci Nano</addtitle><description>We aim to establish the effect of environmental diversity in evaluating nanotoxicity to bacteria. We assessed the toxicity of 4 nm polyallylamine hydrochloride-wrapped gold nanoparticles (PAH AuNPs) to a panel of bacteria from diverse environmental niches. The bacteria experienced a range of toxicities as evidenced by the different minimum bactericidal concentrations determined; the sensitivities of the bacteria was
A. vinelandii
=
P. aeruginosa
>
S. oneidensis
MR-4 >
A. baylyi
>
S. oneidensis
MR-1. Interactions between gold nanoparticles and molecular components of the cell wall were investigated by TEM, flow cytometry, and computational modeling. Binding results showed a general trend that bacteria with smooth lipopolysaccharides (LPS) bind more PAH AuNPs than bacteria with rough LPS. Computational models reveal that PAH migrates to phosphate groups in the core of the LPS structure. Overall, our results demonstrate that simple interactions between nanoparticles and the bacterial cell wall cannot fully account for observed trends in toxicity, which points to the importance of establishing more comprehensive approaches for modeling environmental nanotoxicity.
We aim to establish the effect of bacterial diversity in assessing nanotoxicity.</description><subject>Bacteria</subject><subject>Computation</subject><subject>Computer applications</subject><subject>Cytometry</subject><subject>Environment models</subject><subject>Environmental effects</subject><subject>Flow cytometry</subject><subject>Gold</subject><subject>Gram-negative bacteria</subject><subject>Interactions</subject><subject>Lipopolysaccharides</subject><subject>Mathematical models</subject><subject>Modelling</subject><subject>Nanoparticles</subject><subject>Niches</subject><subject>Phosphates</subject><subject>Polyallylamine</subject><subject>Polyallylamine hydrochloride</subject><subject>Toxicity</subject><issn>2051-8153</issn><issn>2051-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkk1v1DAQhiMEotXSC3eQJS4IKeCPjRNfkKrVtiBVcKHnaOJMdl05drC9C_kd_OF6u2X5OHmsefxq3nldFC8Zfc-oUB90jY7SRnB8UpxzWrGyYZI9PdWVOCsuYryjlDLGKyHr58UZVw2taiXOi1-30bgNAUfQ7U3wbkSXwNq5DGhxDy6RCRxa4gdyHWAsHW4gmT2SDnTCYIAkTyBGjJGkLebbT6NNmg8PJm_ng5aF0Tgk27kPXm-tD6bH8keAacKebLztiQPnJwjJaIvxRfFsABvx4vFcFLdX62-rT-XN1-vPq8ubUi-lSiXUQ697RhU0XUU1RV3XlZSygwb7SisuBGa7wJHpWinJl3LgPeuWoKjoQIpF8fGoO-26EXudnQew7RTMCGFuPZj2344z23bj922lpOBZZFG8fRQI_vsOY2pHEzVamzfmd7HldCkpazgVGX3zH3rnd8Fle5litJG1eqDeHSkdfIwBh9MwjLaHuNtVvf7yEPc6w6__Hv-E_g43A6-OQIj61P3zX8Q9712zzg</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Buchman, Joseph T</creator><creator>Rahnamoun, Ali</creator><creator>Landy, Kaitlin M</creator><creator>Zhang, Xi</creator><creator>Vartanian, Ariane M</creator><creator>Jacob, Lisa M</creator><creator>Murphy, Catherine J</creator><creator>Hernandez, Rigoberto</creator><creator>Haynes, Christy L</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7ST</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5827-8513</orcidid><orcidid>https://orcid.org/0000-0001-7066-5575</orcidid><orcidid>https://orcid.org/0000-0002-5420-5867</orcidid><orcidid>https://orcid.org/0000-0002-8275-8469</orcidid><orcidid>https://orcid.org/0000-0001-8526-7414</orcidid></search><sort><creationdate>20180201</creationdate><title>Using an environmentally-relevant panel of Gram-negative bacteria to assess the toxicity of polyallylamine hydrochloride-wrapped gold nanoparticles</title><author>Buchman, Joseph T ; Rahnamoun, Ali ; Landy, Kaitlin M ; Zhang, Xi ; Vartanian, Ariane M ; Jacob, Lisa M ; Murphy, Catherine J ; Hernandez, Rigoberto ; Haynes, Christy L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-a7fdcd109a8b50c0ec775666ba8ed5c9233e367a2e1c7996246f2d1b4a903ba63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Bacteria</topic><topic>Computation</topic><topic>Computer applications</topic><topic>Cytometry</topic><topic>Environment models</topic><topic>Environmental effects</topic><topic>Flow cytometry</topic><topic>Gold</topic><topic>Gram-negative bacteria</topic><topic>Interactions</topic><topic>Lipopolysaccharides</topic><topic>Mathematical models</topic><topic>Modelling</topic><topic>Nanoparticles</topic><topic>Niches</topic><topic>Phosphates</topic><topic>Polyallylamine</topic><topic>Polyallylamine hydrochloride</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Buchman, Joseph T</creatorcontrib><creatorcontrib>Rahnamoun, Ali</creatorcontrib><creatorcontrib>Landy, Kaitlin M</creatorcontrib><creatorcontrib>Zhang, Xi</creatorcontrib><creatorcontrib>Vartanian, Ariane M</creatorcontrib><creatorcontrib>Jacob, Lisa M</creatorcontrib><creatorcontrib>Murphy, Catherine J</creatorcontrib><creatorcontrib>Hernandez, Rigoberto</creatorcontrib><creatorcontrib>Haynes, Christy L</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Environmental science. Nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buchman, Joseph T</au><au>Rahnamoun, Ali</au><au>Landy, Kaitlin M</au><au>Zhang, Xi</au><au>Vartanian, Ariane M</au><au>Jacob, Lisa M</au><au>Murphy, Catherine J</au><au>Hernandez, Rigoberto</au><au>Haynes, Christy L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Using an environmentally-relevant panel of Gram-negative bacteria to assess the toxicity of polyallylamine hydrochloride-wrapped gold nanoparticles</atitle><jtitle>Environmental science. Nano</jtitle><addtitle>Environ Sci Nano</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>5</volume><issue>2</issue><spage>279</spage><epage>288</epage><pages>279-288</pages><issn>2051-8153</issn><eissn>2051-8161</eissn><abstract>We aim to establish the effect of environmental diversity in evaluating nanotoxicity to bacteria. We assessed the toxicity of 4 nm polyallylamine hydrochloride-wrapped gold nanoparticles (PAH AuNPs) to a panel of bacteria from diverse environmental niches. The bacteria experienced a range of toxicities as evidenced by the different minimum bactericidal concentrations determined; the sensitivities of the bacteria was
A. vinelandii
=
P. aeruginosa
>
S. oneidensis
MR-4 >
A. baylyi
>
S. oneidensis
MR-1. Interactions between gold nanoparticles and molecular components of the cell wall were investigated by TEM, flow cytometry, and computational modeling. Binding results showed a general trend that bacteria with smooth lipopolysaccharides (LPS) bind more PAH AuNPs than bacteria with rough LPS. Computational models reveal that PAH migrates to phosphate groups in the core of the LPS structure. Overall, our results demonstrate that simple interactions between nanoparticles and the bacterial cell wall cannot fully account for observed trends in toxicity, which points to the importance of establishing more comprehensive approaches for modeling environmental nanotoxicity.
We aim to establish the effect of bacterial diversity in assessing nanotoxicity.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>29805793</pmid><doi>10.1039/c7en00832e</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-5827-8513</orcidid><orcidid>https://orcid.org/0000-0001-7066-5575</orcidid><orcidid>https://orcid.org/0000-0002-5420-5867</orcidid><orcidid>https://orcid.org/0000-0002-8275-8469</orcidid><orcidid>https://orcid.org/0000-0001-8526-7414</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Environmental science. Nano, 2018-02, Vol.5 (2), p.279-288 |
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| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5963290 |
| source | Royal Society of Chemistry |
| subjects | Bacteria Computation Computer applications Cytometry Environment models Environmental effects Flow cytometry Gold Gram-negative bacteria Interactions Lipopolysaccharides Mathematical models Modelling Nanoparticles Niches Phosphates Polyallylamine Polyallylamine hydrochloride Toxicity |
| title | Using an environmentally-relevant panel of Gram-negative bacteria to assess the toxicity of polyallylamine hydrochloride-wrapped gold nanoparticles |
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