HKOCl-3: a fluorescent hypochlorous acid probe for live-cell and in vivo imaging and quantitative application in flow cytometry and a 96-well microplate assay† †Electronic supplementary information (ESI) available: Experimental procedures, characterization data and additional spectra. See DOI: 10.1039/c5sc03855c

• Published in: Chemical science (Cambridge) 2016-01, Vol.7 (3), p.2094-2099
• Main Authors: Hu, Jun Jacob, Wong, Nai-Kei, Lu, Ming-Yang, Chen, Xingmiao, Ye, Sen, Zhao, Angela Qian, Gao, Peng, Yi-Tsun Kao, Richard, Shen, Jiangang, Yang, Dan
• Format: Article
• Language: English
• Subjects: Chemistry
• ISSN: 2041-6520; 2041-6539
• DOI: 10.1039/c5sc03855c

We describe an ultra-selective and ultra-sensitive HOCI fluorescent probe HKOCl-3 for live-cell and in vivo imaging and quantitative applications. Ultra-selective and ultra-sensitive probes for hypochlorous acid (HOCl), one of the most poorly understood reactive oxygen species (ROS), are urgently needed to unravel the HOCl functions in important biological processes such as development and innate immunity. Based on a selective oxidative O -dearylation reaction of 2,6-dichlorophenol toward HOCl over other reactive oxygen species, we have developed a novel fluorescent probe HKOCl-3 for HOCl detection with ultra-selectivity, ultra-sensitivity and a rapid turn-on response. The functional robustness of HKOCl-3 for endogenous HOCl detection and imaging has been thoroughly scrutinized in multiple types of phagocytes and in vivo imaging of live intact zebrafish embryos. Furthermore, HKOCl-3 has been successfully applied to the detection of endogenous HOCl by a 96-well microplate assay and flow cytometry. Therefore, HKOCl-3 holds great promise as a versatile molecular tool that enables innovative investigation of HOCl biology and ROS-related diseases in multiple detection modalities.