Persistence of Long-lived Memory B Cells specific to Duffy Binding Protein in individuals exposed to Plasmodium vivax

The major challenge in designing a protective Duffy binding protein region II (DBPII)-based vaccine against blood-stage vivax malaria is the high number of polymorphisms in critical residues targeted by binding-inhibitory antibodies. Here, longevity of antibody and memory B cell response (MBCs) to D...

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Bibliographic Details
Published in:Scientific reports 2018-05, Vol.8 (1), p.8347-11, Article 8347
Main Authors: Changrob, Siriruk, McHenry, Amy M., Nyunt, Myat Htut, Sattabongkot, Jetsumon, Han, Eun-Taek, Adams, John H., Chootong, Patchanee
Format: Article
Language:English
Subjects:
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Antibody response
Antigens
Disease transmission
Helper cells
Humanities and Social Sciences
Immunological memory
Infections
Lymphocytes B
Lymphocytes T
Malaria
multidisciplinary
Phenotyping
Science
Science (multidisciplinary)
Vector-borne diseases
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Summary:The major challenge in designing a protective Duffy binding protein region II (DBPII)-based vaccine against blood-stage vivax malaria is the high number of polymorphisms in critical residues targeted by binding-inhibitory antibodies. Here, longevity of antibody and memory B cell response (MBCs) to DBL-TH variants, DBL-TH2, -TH4, -TH5, -TH6 and -TH9 were analyzed in P. vivax -exposed individuals living in a low malaria transmission area of southern Thailand. Antibody to DBL-TH variants were significantly detected during P. vivax infection and it was persisted for up to 9 months post-infection. However, DBL-TH-specific MBC responses were stably maintained longer than antibody response, at least 3 years post-infection in the absence of re-infection. Phenotyping of B cell subsets showed the expansion of activated and atypical MBCs during acute and recovery phase of infection. While the persistence of DBL-TH-specific MBCs was found in individuals who had activated and atypical MBC expansion, anti-DBL-TH antibody responses was rapidly declined in plasma. The data suggested that these two MBCs were triggered by P. vivax infection, its expansion and stability may have impact on antibody responses. Our results provided evidence for ability of DBPII variant antigens in induction of long-lasting MBCs among individuals who were living in low malaria endemicity.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-26677-x