Serine Protease Inhibitor SERPINE2 Reversibly Modulates Murine Sperm Capacitation

SERPINE2 (serpin peptidase inhibitor, clade E, member 2), predominantly expressed in the seminal vesicle, can inhibit murine sperm capacitation, suggesting its role as a sperm decapacitation factor (DF). A characteristic of DF is its ability to reverse the capacitation process. Here, we investigated...

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Published in:International journal of molecular sciences 2018-05, Vol.19 (5), p.1520
Main Authors: Li, Sheng-Hsiang, Hwu, Yuh-Ming, Lu, Chung-Hao, Lin, Ming-Huei, Yeh, Ling-Yu, Lee, Robert Kuo-Kuang
Format: Article
Language:English
Subjects:
Acrosome - drug effects
Acrosome - metabolism
Acrosome Reaction
Animals
Calcimycin
Calcium
Calcium - metabolism
Calcium ions
Capacitation
Male
Mice
Mice, Inbred ICR
Oviduct
Peptidase
Phosphorylation
Protease inhibitors
Proteinase inhibitors
Proteins
Seminal vesicle
Serine
Serine proteinase
Serpin E2 - metabolism
Serpin E2 - pharmacology
Sperm
Tyrosine
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Summary:SERPINE2 (serpin peptidase inhibitor, clade E, member 2), predominantly expressed in the seminal vesicle, can inhibit murine sperm capacitation, suggesting its role as a sperm decapacitation factor (DF). A characteristic of DF is its ability to reverse the capacitation process. Here, we investigated whether SERPINE2 can reversibly modulate sperm capacitation. Immunocytochemical staining revealed that SERPINE2 was bound onto both capacitated and uncapacitated sperm. It reversed the increase in BSA-induced sperm protein tyrosine phosphorylation levels. The effective dose and incubation time were found to be >0.1 mg/mL and >60 min, respectively. Calcium ion levels in the capacitated sperm were reduced to a level similar to that in uncapacitated sperm after 90 min of incubation with SERPINE2. In addition, the acrosome reaction of capacitated sperm was inhibited after 90 min of incubation with SERPINE2. Oviductal sperm was readily induced to undergo the acrosome reaction using the A23187 ionophore; however, the acrosome reaction was significantly reduced after incubation with SERPINE2 for 60 and 120 min. These findings suggested that SERPINE2 prevented as well as reversed sperm capacitation in vitro. It also prevented the acrosome reaction in in vivo-capacitated sperm isolated from the oviduct. Thus, SERPINE2 could reversibly modulate murine sperm capacitation.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms19051520