AtPep3 is a hormone-like peptide that plays a role in the salinity stress tolerance of plants

• Published in: Proceedings of the National Academy of Sciences - PNAS 2018-05, Vol.115 (22), p.5810-5815
• Main Authors: Nakaminami, Kentaro, Okamoto, Masanori, Higuchi-Takeuchi, Mieko, Yoshizumi, Takeshi, Yamaguchi, Yube, Fukao, Yoichiro, Shimizu, Minami, Ohashi, Chihiro, Tanaka, Maho, Matsui, Minami, Shinozaki, Kazuo, Seki, Motoaki, Hanada, Kousuke
• Format: Article
• Language: English
• Subjects: Abiotic stress; Amino acids; Arabidopsis; Arabidopsis - genetics; Arabidopsis - physiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - physiology; Biological Sciences; Bleaching; Chlorophyll; Genes; Genes, Plant - genetics; Immune response; Immune system; Immunological tolerance; Peptide Hormones - genetics; Peptide Hormones - physiology; Peptides; Phenotypes; Plant growth; Plant hormones; Plants, Genetically Modified - genetics; Plants, Genetically Modified - physiology; Salinity; Salinity effects; Salt-Tolerance - genetics; Salt-Tolerance - physiology; Seedlings; Seedlings - genetics; Seedlings - physiology; Stress; Stress, Physiological - genetics; Stresses; Survivability; Synthetic peptides; Transgenic plants
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1719491115

Peptides encoded by small coding genes play an important role in plant development, acting in a similar manner as phytohormones. Few hormone-like peptides, however, have been shown to play a role in abiotic stress tolerance. In the current study, 17 Arabidopsis genes coding for small peptides were found to be up-regulated in response to salinity stress. To identify peptides leading salinity stress tolerance, we generated transgenic Arabidopsis plants overexpressing these small coding genes and assessed survivability and root growth under salinity stress conditions. Results indicated that 4 of the 17 overexpressed genes increased salinity stress tolerance. Further studies focused on AtPROPEP3, which was the most highly up-regulated gene under salinity stress. Treatment of plants with synthetic peptides encoded by AtPROPEP3 revealed that a C-terminal peptide fragment (AtPep3) inhibited the salt-induced bleaching of chlorophyll in seedlings. Conversely, knockdown AtPROPEP3 transgenic plants exhibited a hypersensitive phenotype under salinity stress, which was complemented by the AtPep3 peptide. This functional AtPep3 peptide region overlaps with an AtPep3 elicitor peptide that is related to the immune response of plants. Functional analyses with a receptor mutant of AtPep3 revealed that AtPep3 was recognized by the PEPR1 receptor and that it functions to increase salinity stress tolerance in plants. Collectively, these data indicate that AtPep3 plays a significant role in both salinity stress tolerance and immune response in Arabidopsis.