A gender specific improved survival related to stromal miR-143 and miR-145 expression in non-small cell lung cancer

Micro RNAs (miRNA) are small non-coding RNAs that post-transcriptionally regulate gene expression. Dysregulation of miRNA cluster 143/145 has been reported in several malignancies, but their role in non-small cell lung cancer (NSCLC) remains elusive. This study investigates the prognostic impact of...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2018-06, Vol.8 (1), p.8549-12, Article 8549
Main Authors: Skjefstad, Kaja, Johannessen, Charles, Grindstad, Thea, Kilvaer, Thomas, Paulsen, Erna-Elise, Pedersen, Mona, Donnem, Tom, Andersen, Sigve, Bremnes, Roy, Richardsen, Elin, Al-Saad, Samer, Busund, Lill-Tove
Format: Article
Language:English
Subjects:
13/105
14/63
45/77
631/67/1612/1350
692/4028/67/1612/1350
96/109
Clinical medical disciplines: 750
DNA microarrays
Epithelial cells
Gene expression
Humanities and Social Sciences
Klinisk medisinske fag: 750
Lung cancer
Lymph nodes
Lymphatic system
Medical disciplines: 700
Medisinske Fag: 700
Metastases
Metastasis
miRNA
multidisciplinary
Non-small cell lung carcinoma
Oncology: 762
Onkologi: 762
Post-transcription
Science
Science (multidisciplinary)
Steroid hormone receptors
Stromal cells
Tumorigenesis
Tumors
VDP
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Micro RNAs (miRNA) are small non-coding RNAs that post-transcriptionally regulate gene expression. Dysregulation of miRNA cluster 143/145 has been reported in several malignancies, but their role in non-small cell lung cancer (NSCLC) remains elusive. This study investigates the prognostic impact of miR-143 and miR-145 in primary tumors and metastatic lymph nodes in NSCLC tissue. Tissue from 553 primary tumors and 143 matched metastatic lymph nodes were collected and tissue microarrays were constructed. In situ hybridization was used to evaluate miR-143 and miR-145 expression in tumor epithelial cells and stromal cells in the primary tumors and lymph nodes. In vivo data was supplemented with functional studies of cell lines in vitro to evaluate the role of miR-143 and miR-145 in NSCLC tumorigenesis. In our cohort, stromal miR-143 (S-miR-143) and miR-145 (S-miR-145) expression in primary tumor tissue were independent prognosticators of improved disease-specific survival (DSS) in female (S-miR-143, HR: 0.53, p = 0.019) and male patients (S-miR-145, HR: 0.58, p = 0.021), respectively. Interesting correlations between the miR cluster 143/145 and previously investigated steroid hormone receptors from the same cohort were identified, substantiating their gender dependent significance.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-26864-w