Set up of a protocol for rat plasma peptidomics in hemorrhagic shock model in presence of heparin

[Display omitted] •A protocol for peptidome plasma analysis has been set up.•The presence of heparin did not affect peptides detection in plasma specimens.•Plasma displays an increase in peptides after hemorrhagic shock. A preliminary mass spectrometry based shotgun protocol was set up to compare th...

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Bibliographic Details
Published in:EuPA open proteomics 2016-09, Vol.12, p.1-3
Main Authors: Maffioli, Elisa, Aletti, Federico, Santagata, Fabiana, Negri, Armando, Santamaria, Marco H., De Lano, Frank A., Kistler, Erik B., Schmid-Schönbein, Geert W., Tedeschi, Gabriella
Format: Article
Language:English
Subjects:
Hemorrhagic shock
Heparin
Mass spectrometry
Peptidomics
Proteolysis
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Summary:[Display omitted] •A protocol for peptidome plasma analysis has been set up.•The presence of heparin did not affect peptides detection in plasma specimens.•Plasma displays an increase in peptides after hemorrhagic shock. A preliminary mass spectrometry based shotgun protocol was set up to compare the peptidome of plasma samples from healthy and hemorrhagic shock rats with the aim of verifying the possible role of uncontrolled proteolytic activity in circulatory shock. Since the hemorrhagic shock model requires heparin as anticoagulant, a preliminary experiment using plasma sample obtained in the presence/absence of heparin from healthy rats was performed to determine whether its presence is fully compatible with the peptidomic protocol proposed. The entire protocol was tested in a pilot experiment to compare the peptidome of healthy or heparin-anticoagulated rats subjected to hemorrhagic shock.
ISSN:2212-9685
2212-9685
DOI:10.1016/j.euprot.2016.03.004