Effects of ozone stimulation of bronchial epithelial cells on proliferation and collagen synthesis of co-cultured lung fibroblasts

Ozone (O ) as a major air pollutant is widely recognized for causing pathological changes of the airway system. However, it is not clear whether O exposure of bronchial epithelial cells (BECs) influences the proliferation and collagen synthesis of submucosal fibroblasts and contributes to the pathog...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2018-06, Vol.15 (6), p.5314-5322
Main Authors: Wang, Yue, Tan, Meiling, Ouyang, Haiping, Deng, Linhong
Format: Article
Language:English
Subjects:
Air pollution
Asthma
Bronchi
Cell growth
Cell proliferation
Collagen
Cytokines
Development and progression
Epithelial cells
Fibroblasts
Health aspects
Inflammation
Kinases
Ozone
Physiological aspects
Smooth muscle
Stainless steel
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Summary:Ozone (O ) as a major air pollutant is widely recognized for causing pathological changes of the airway system. However, it is not clear whether O exposure of bronchial epithelial cells (BECs) influences the proliferation and collagen synthesis of submucosal fibroblasts and contributes to the pathogenesis of airway remodeling in diseases, including asthma. In the present study, a co-culture method was applied to culture human lung fibroblasts (HLFs) with human bronchial epithelial cells (HBECs) that were pre-stimulated with O . Following co-culture for up to 24 h, the proliferation of HLFs was measured using MTT colorimetry. Furthermore, the collagen synthesis capacity of HLFs was determined by the level of hydroxyproline. In addition, the protein expression levels of cytokines, including transforming growth factor (TGF)-β1, tumor necrosis factor (TNF)-α and prostaglandin E2 (PGE2) were assessed. Results indicated that the proliferation of HLFs co-cultured with HBECs was significantly inhibited when compared with HLFs cultured alone (P
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2018.6122