Exogenous Tissue Inhibitor of Metalloproteinase-2 Affects Matrix Metalloproteinase-2 Expression in Conjunctival Filtering Blebs and Bleb Scarring in Rats

Objective. To examine the effect of tissue inhibitor of metalloproteinase-2 (TIMP-2) on conjunctival filtering bleb scarring. Methods. A model of conjunctival filtering bleb was established whereby rats were injected with saline, blank adenoviral vector, or adenoviral vector carrying TIMP-2 into the...

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Published in:BioMed research international 2018-01, Vol.2018 (2018), p.1-8
Main Authors: Li, Na, Yin, Gang, Liu, Meng-ying, Wang, Ling, Wang, Dabo
Format: Article
Language:English
Subjects:
Adenoviruses
Animals
Biomedical research
Blister - metabolism
Cicatrix
Conjunctiva - metabolism
Epithelium
Fibroblasts
Filtration
Gelatinase A
Gene expression
Glaucoma
Infection
Inhibitors
Kinases
Lamina propria
Mathematical analysis
Matrix metalloproteinase
Matrix Metalloproteinase 2 - metabolism
Matrix methods
Metalloproteinase
Morphology
Protein expression
Proteins
Rats
Rodents
Scars
Science
Success
Surgery
Tissue inhibitor of metalloproteinase 2
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2 - physiology
Transfection
Wound healing
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Yin, Gang
Liu, Meng-ying
Wang, Ling
Wang, Dabo
description Objective. To examine the effect of tissue inhibitor of metalloproteinase-2 (TIMP-2) on conjunctival filtering bleb scarring. Methods. A model of conjunctival filtering bleb was established whereby rats were injected with saline, blank adenoviral vector, or adenoviral vector carrying TIMP-2 into the bleb. Filtration bleb formation and matrix metalloproteinase-2 (MMP-2) expression were examined. Results. All operated eyes formed obvious elevated blebs on day 1. In the normal saline group, empty plasmid group, and gene transfection group maintenance time of filtrating blebs was 5–14, 5–14, and 6–16 days, and average survival time was 8.24, 8.16, and 9.44 days, respectively. MMP-2 expression increased slightly in the gene transfection group at 3 and 5 days after surgery, reached a peak after 14 days, and then gradually decreased. MMP-2 expression was weakly positive in the normal conjunctival epithelium, but was hardly detected in the lamina propria. Seven days after surgery, the epithelium and lamina propria of the conjunctival filtering bleb exhibited strong positive expression in the empty plasmid group but only weak expression in the adenovirus group. Conclusion. Exogenous TIMP-2 interfered with local MMP-2 expression, delaying peak expression of MMP-2 and slowing the scarring of filtering blebs during wound healing of subconjunctival tissue.
doi_str_mv 10.1155/2018/9365950
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To examine the effect of tissue inhibitor of metalloproteinase-2 (TIMP-2) on conjunctival filtering bleb scarring. Methods. A model of conjunctival filtering bleb was established whereby rats were injected with saline, blank adenoviral vector, or adenoviral vector carrying TIMP-2 into the bleb. Filtration bleb formation and matrix metalloproteinase-2 (MMP-2) expression were examined. Results. All operated eyes formed obvious elevated blebs on day 1. In the normal saline group, empty plasmid group, and gene transfection group maintenance time of filtrating blebs was 5–14, 5–14, and 6–16 days, and average survival time was 8.24, 8.16, and 9.44 days, respectively. MMP-2 expression increased slightly in the gene transfection group at 3 and 5 days after surgery, reached a peak after 14 days, and then gradually decreased. MMP-2 expression was weakly positive in the normal conjunctival epithelium, but was hardly detected in the lamina propria. Seven days after surgery, the epithelium and lamina propria of the conjunctival filtering bleb exhibited strong positive expression in the empty plasmid group but only weak expression in the adenovirus group. Conclusion. Exogenous TIMP-2 interfered with local MMP-2 expression, delaying peak expression of MMP-2 and slowing the scarring of filtering blebs during wound healing of subconjunctival tissue.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2018/9365950</identifier><identifier>PMID: 29955613</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Adenoviruses ; Animals ; Biomedical research ; Blister - metabolism ; Cicatrix ; Conjunctiva - metabolism ; Epithelium ; Fibroblasts ; Filtration ; Gelatinase A ; Gene expression ; Glaucoma ; Infection ; Inhibitors ; Kinases ; Lamina propria ; Mathematical analysis ; Matrix metalloproteinase ; Matrix Metalloproteinase 2 - metabolism ; Matrix methods ; Metalloproteinase ; Morphology ; Protein expression ; Proteins ; Rats ; Rodents ; Scars ; Science ; Success ; Surgery ; Tissue inhibitor of metalloproteinase 2 ; Tissue Inhibitor of Metalloproteinase-1 ; Tissue Inhibitor of Metalloproteinase-2 - physiology ; Transfection ; Wound healing</subject><ispartof>BioMed research international, 2018-01, Vol.2018 (2018), p.1-8</ispartof><rights>Copyright © 2018 Ling Wang et al.</rights><rights>COPYRIGHT 2018 John Wiley &amp; Sons, Inc.</rights><rights>Copyright © 2018 Ling Wang et al.; This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2018 Ling Wang et al. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-404a288d9726fe2daa80de9a091205c604d3d31c794e6f15c69c7a0a705da1ab3</citedby><cites>FETCH-LOGICAL-c499t-404a288d9726fe2daa80de9a091205c604d3d31c794e6f15c69c7a0a705da1ab3</cites><orcidid>0000-0002-7871-7330 ; 0000-0003-1736-7880 ; 0000-0002-7676-3181</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2052737646/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2052737646?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,25732,27903,27904,36991,36992,44569,74872</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29955613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Nakazawa, Mitsuru</contributor><contributor>Mitsuru Nakazawa</contributor><creatorcontrib>Li, Na</creatorcontrib><creatorcontrib>Yin, Gang</creatorcontrib><creatorcontrib>Liu, Meng-ying</creatorcontrib><creatorcontrib>Wang, Ling</creatorcontrib><creatorcontrib>Wang, Dabo</creatorcontrib><title>Exogenous Tissue Inhibitor of Metalloproteinase-2 Affects Matrix Metalloproteinase-2 Expression in Conjunctival Filtering Blebs and Bleb Scarring in Rats</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Objective. To examine the effect of tissue inhibitor of metalloproteinase-2 (TIMP-2) on conjunctival filtering bleb scarring. Methods. A model of conjunctival filtering bleb was established whereby rats were injected with saline, blank adenoviral vector, or adenoviral vector carrying TIMP-2 into the bleb. Filtration bleb formation and matrix metalloproteinase-2 (MMP-2) expression were examined. Results. All operated eyes formed obvious elevated blebs on day 1. In the normal saline group, empty plasmid group, and gene transfection group maintenance time of filtrating blebs was 5–14, 5–14, and 6–16 days, and average survival time was 8.24, 8.16, and 9.44 days, respectively. MMP-2 expression increased slightly in the gene transfection group at 3 and 5 days after surgery, reached a peak after 14 days, and then gradually decreased. MMP-2 expression was weakly positive in the normal conjunctival epithelium, but was hardly detected in the lamina propria. 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Exogenous TIMP-2 interfered with local MMP-2 expression, delaying peak expression of MMP-2 and slowing the scarring of filtering blebs during wound healing of subconjunctival tissue.</description><subject>Adenoviruses</subject><subject>Animals</subject><subject>Biomedical research</subject><subject>Blister - metabolism</subject><subject>Cicatrix</subject><subject>Conjunctiva - metabolism</subject><subject>Epithelium</subject><subject>Fibroblasts</subject><subject>Filtration</subject><subject>Gelatinase A</subject><subject>Gene expression</subject><subject>Glaucoma</subject><subject>Infection</subject><subject>Inhibitors</subject><subject>Kinases</subject><subject>Lamina propria</subject><subject>Mathematical analysis</subject><subject>Matrix metalloproteinase</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Matrix methods</subject><subject>Metalloproteinase</subject><subject>Morphology</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rodents</subject><subject>Scars</subject><subject>Science</subject><subject>Success</subject><subject>Surgery</subject><subject>Tissue inhibitor of metalloproteinase 2</subject><subject>Tissue Inhibitor of Metalloproteinase-1</subject><subject>Tissue Inhibitor of Metalloproteinase-2 - physiology</subject><subject>Transfection</subject><subject>Wound healing</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqNksFuEzEQhlcIRKvSG2dkiQsShNper3d9QUqjFCq1QoJytibe2cTRxg62t4RH6dvWaUIoiAO-eDTz-R_P6C-Kl4y-Z6yqzjhlzZkqZaUq-qQ45iUTI8kEe3qIy_KoOI1xSfNpmKRKPi-OuFJVlWvHxd104-fo_BDJjY1xQHLpFnZmkw_Ed-QaE_S9Xwef0DqIOOJk3HVoUiTXkILd_BOZbtYBY7TeEevIxLvl4Eyyt9CTC9snDNbNyXmPs0jAtQ8R-WogPOTziy-Q4oviWQd9xNP9fVJ8u5jeTD6Nrj5_vJyMr0ZGKJVGggrgTdOqmssOeQvQ0BYVUMU4rYykoi3bkplaCZQdyxllaqBQ06oFBrPypPiw010PsxW2Bl0K0Ot1sCsIP7UHq_-sOLvQc3-r5XajQmSBN3uB4L8PGJNe2Wiw78Fh3qvmVPKmpJzLjL7-C136Ibg8XqYqXpe1FI-oOfSoret87mu2onosOauVEpXK1LsdZYKPMWB3-DKjemsOvTWH3psj468ej3mAf1khA293wMK6Fn7Y_5TDzGAHv2nGVU1peQ_GS8yD</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Li, Na</creator><creator>Yin, Gang</creator><creator>Liu, Meng-ying</creator><creator>Wang, Ling</creator><creator>Wang, Dabo</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley &amp; 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To examine the effect of tissue inhibitor of metalloproteinase-2 (TIMP-2) on conjunctival filtering bleb scarring. Methods. A model of conjunctival filtering bleb was established whereby rats were injected with saline, blank adenoviral vector, or adenoviral vector carrying TIMP-2 into the bleb. Filtration bleb formation and matrix metalloproteinase-2 (MMP-2) expression were examined. Results. All operated eyes formed obvious elevated blebs on day 1. In the normal saline group, empty plasmid group, and gene transfection group maintenance time of filtrating blebs was 5–14, 5–14, and 6–16 days, and average survival time was 8.24, 8.16, and 9.44 days, respectively. MMP-2 expression increased slightly in the gene transfection group at 3 and 5 days after surgery, reached a peak after 14 days, and then gradually decreased. MMP-2 expression was weakly positive in the normal conjunctival epithelium, but was hardly detected in the lamina propria. Seven days after surgery, the epithelium and lamina propria of the conjunctival filtering bleb exhibited strong positive expression in the empty plasmid group but only weak expression in the adenovirus group. Conclusion. Exogenous TIMP-2 interfered with local MMP-2 expression, delaying peak expression of MMP-2 and slowing the scarring of filtering blebs during wound healing of subconjunctival tissue.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>29955613</pmid><doi>10.1155/2018/9365950</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-7871-7330</orcidid><orcidid>https://orcid.org/0000-0003-1736-7880</orcidid><orcidid>https://orcid.org/0000-0002-7676-3181</orcidid><oa>free_for_read</oa></addata></record>
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subjects Adenoviruses
Animals
Biomedical research
Blister - metabolism
Cicatrix
Conjunctiva - metabolism
Epithelium
Fibroblasts
Filtration
Gelatinase A
Gene expression
Glaucoma
Infection
Inhibitors
Kinases
Lamina propria
Mathematical analysis
Matrix metalloproteinase
Matrix Metalloproteinase 2 - metabolism
Matrix methods
Metalloproteinase
Morphology
Protein expression
Proteins
Rats
Rodents
Scars
Science
Success
Surgery
Tissue inhibitor of metalloproteinase 2
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2 - physiology
Transfection
Wound healing
title Exogenous Tissue Inhibitor of Metalloproteinase-2 Affects Matrix Metalloproteinase-2 Expression in Conjunctival Filtering Blebs and Bleb Scarring in Rats
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