Sucrose assists selection of high‐quality oocytes in pigs

We investigated whether high‐quality in vitro matured (IVM) oocytes can be distinguished from poor ones based on the morphological changes after treatment with hyperosmotic medium containing 0.2 mol/L sucrose in pigs. We hypothesize that IVM oocytes maintaining round shape have higher quality than m...

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Bibliographic Details
Published in:Animal science journal 2018-06, Vol.89 (6), p.880-887
Main Authors: Dang‐Nguyen, Thanh Quang, Nguyen, Hiep Thi, Somfai, Tamas, Wells, David, Men, Nguyen Thi, Viet‐Linh, Nguyen, Noguchi, Junko, Kaneko, Hiroyuki, Kikuchi, Kazuhiro, Nagai, Takashi
Format: Article
Language:English
Subjects:
Actin
Actin Cytoskeleton
Animals
Blastocyst
Blastocysts
Cell Count
Cell culture
Cell number
Culture Media - pharmacology
Cytoskeleton
Dehydration
Embryonic Development - drug effects
embryonic development competence
Embryos
Female
Fertilization in Vitro
In vitro fertilization
In Vitro Oocyte Maturation Techniques
Microfilaments
Microtubules
Nuclear transfer
Nuclear Transfer Techniques
oocyte quality
Oocytes
Oocytes - cytology
Oocytes - growth & development
Original
ORIGINAL ARTICLES
pig
Quality
Sucrose
Sucrose - pharmacology
Sugar
Swine
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Summary:We investigated whether high‐quality in vitro matured (IVM) oocytes can be distinguished from poor ones based on the morphological changes after treatment with hyperosmotic medium containing 0.2 mol/L sucrose in pigs. We hypothesize that IVM oocytes maintaining round shape have higher quality than mis‐shapened oocytes following dehydration. Oocyte quality was verified by determining embryonic developmental competence using in vitro fertilization, nuclear transfer and parthenogenetic activation. In all cases, the round oocytes had greater (p 
ISSN:1344-3941
1740-0929
DOI:10.1111/asj.13015