Effect of ultraviolet light treatment on surface hydrophilicity and human gingival fibroblast response on nanostructured titanium surfaces

This study was designed to investigate the effect of nanostructured TiO2 coatings on human gingival fibroblast and to explore the influence of ultraviolet (UV) light on surface wettability and cellular response. Ti‐6Al‐4V titanium alloy discs (n = 96) were divided into three groups: a sol–gel‐derive...

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Bibliographic Details
Published in:Clinical and experimental dental research 2018-06, Vol.4 (3), p.78-85
Main Authors: Areid, Nagat, Peltola, Ari, Kangasniemi, Ilkka, Ballo, Ahmed, Närhi, Timo O.
Format: Article
Language:English
Subjects:
Biocompatibility
Biofilms
Biological activity
contact angle
Dental implants
Dentistry
fibroblast
Fibroblasts
Methods
nanostructure
Original
Studies
TiO2
Titanium alloys
Topography
Transplants & implants
UV light
Wound healing
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Summary:This study was designed to investigate the effect of nanostructured TiO2 coatings on human gingival fibroblast and to explore the influence of ultraviolet (UV) light on surface wettability and cellular response. Ti‐6Al‐4V titanium alloy discs (n = 96) were divided into three groups: a sol–gel‐derived MetAlive™ (MA) coating; hydrothermal (HT) coating; and a non‐coated (NC) group. Forty‐eight titanium substrates were further treated with UV light for 15 min. The water contact angles of the substrates were measured using the sessile drop method. Human gingival fibroblasts were used to evaluate the cell adhesion strength and cell proliferation on experimental surfaces. The strength of cell adhesion against enzymatic detachment was studied after 6 hr of adhesion using gentle trypsinization for 15 min at room temperature. A fluorescence microscope was used for cell imaging (Zeiss‐stereo‐lumar‐v12), and images were analyzed for cell counting, and the percentage of detached cells were calculated. The proliferation of cultured cells up to 10 days was determined according to the cell activity using Alamar Blue™assay. The HT group had the lowest contact angle value (31.1°) followed by MetAlive™ (35.3°), whereas the NC group had the highest contact angle (50.3°). After UV light treatment, all surfaces become considerably more hydrophilic. There was a significant difference in the amount of adherent cells between sol–gel and HT groups when compared with the NC group (p 
ISSN:2057-4347
2057-4347
DOI:10.1002/cre2.108