Cooperative control of Akt phosphorylation, bcl-2 expression, and apoptosis by cytoskeletal microfilaments and microtubules in capillary endothelial cells

Capillary endothelial cells can be switched between growth and apoptosis by modulating their shape with the use of micropatterned adhesive islands. The present study was carried out to examine whether cytoskeletal filaments contribute to this response. Disruption of microfilaments or microtubules wi...

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Bibliographic Details
Published in:Molecular biology of the cell 2001-10, Vol.12 (10), p.3087-3094
Main Authors: Flusberg, D A, Numaguchi, Y, Ingber, D E
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Apoptosis - physiology
Caspases - drug effects
Caspases - metabolism
Cattle
Cell Size - drug effects
Cell Size - physiology
Cell Survival - drug effects
Cell Survival - physiology
Cells, Cultured
Cytochalasin D - pharmacology
Cytoskeleton - drug effects
Cytoskeleton - metabolism
Endothelium, Vascular - cytology
Endothelium, Vascular - metabolism
Microtubules - drug effects
Microtubules - metabolism
Nocodazole - pharmacology
Phosphorylation - drug effects
Protein-Serine-Threonine Kinases
Proto-Oncogene Proteins - drug effects
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins c-akt
Proto-Oncogene Proteins c-bcl-2 - biosynthesis
Proto-Oncogene Proteins c-bcl-2 - drug effects
Space life sciences
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Summary:Capillary endothelial cells can be switched between growth and apoptosis by modulating their shape with the use of micropatterned adhesive islands. The present study was carried out to examine whether cytoskeletal filaments contribute to this response. Disruption of microfilaments or microtubules with the use of cytochalasin D or nocodazole, respectively, led to levels of apoptosis in capillary cells equivalent to that previously demonstrated by inducing cell rounding with the use of micropatterned culture surfaces containing small (
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.12.10.3087