Isolation and Purification of Two Isoflavones from Hericium erinaceum Mycelium by High-Speed Counter-Current Chromatography

High-speed counter-current chromatography (HSCCC) was used to separate and purify two isoflavones for the first time from ( ) mycelium using a two-phase solvent system composed of chloroform-dichloromethane-methanol-water (4:2:3:2, / / / ). These two isoflavones were identified as genistein (4'...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2018-03, Vol.23 (3), p.560
Main Authors: He, Jinzhe, Fan, Peng, Feng, Simin, Shao, Ping, Sun, Peilong
Format: Article
Language:English
Subjects:
Agaricales - chemistry
Chloroform
Chromatography
Countercurrent Distribution - methods
Daidzein
Dichloromethane
Genistein
Genistein - isolation & purification
High speed
Infrared spectroscopy
Ionization
Isoflavones
Isoflavones - isolation & purification
Liquid-Liquid Extraction - methods
Mass spectroscopy
Mycelium - chemistry
NMR
Nuclear magnetic resonance
Purification
Purity
Solvents - chemistry
Spectrum analysis
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Summary:High-speed counter-current chromatography (HSCCC) was used to separate and purify two isoflavones for the first time from ( ) mycelium using a two-phase solvent system composed of chloroform-dichloromethane-methanol-water (4:2:3:2, / / / ). These two isoflavones were identified as genistein (4',5,7-trihydroxyisoflavone, C H O₅) and daidzein (4',7-dihydroxyisoflavone, C H O₄), using infrared spectroscopy (IR), electro-spary ionisation mass (ESI-MS), ¹H-nuclear magnetic resonance (NMR) and C-NMR spectra. About 23 mg genistein with 95.7% purity and 18 mg daidzein with 97.3% purity were isolated from 150 mg ethanolic extract of mycelium. The results demonstrated that HSCCC was a feasible method to separate and purify genistein and daidzein from mycelium.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules23030560