Antimicrobial and Antibiofilm Activity of Human Milk Oligosaccharides against Streptococcus agalactiae, Staphylococcus aureus, and Acinetobacter baumannii

• Published in: ACS infectious diseases 2018-03, Vol.4 (3), p.315-324
• Main Authors: Ackerman, Dorothy L, Craft, Kelly M, Doster, Ryan S, Weitkamp, Jörn-Hendrik, Aronoff, David M, Gaddy, Jennifer A, Townsend, Steven D
• Format: Article
• Language: English
• Subjects: Acinetobacter baumannii - drug effects; Acinetobacter baumannii - growth & development; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Biofilms - drug effects; Biofilms - growth & development; Biological Products - isolation & purification; Biological Products - pharmacology; Female; Humans; Microbial Sensitivity Tests; Milk, Human - chemistry; Oligosaccharides - isolation & purification; Oligosaccharides - pharmacology; Staphylococcus aureus - drug effects; Staphylococcus aureus - growth & development; Streptococcus agalactiae - drug effects; Streptococcus agalactiae - growth & development
• ISSN: 2373-8227; 2373-8227
• DOI: 10.1021/acsinfecdis.7b00183

In a previous study, we reported that human milk oligosaccharides (HMOs) isolated from five donor milk samples possessed antimicrobial and antibiofilm activity against Streptococcus agalactiae, also known as Group B Streptococcus or GBS. Herein, we present a broader evaluation of the antimicrobial and antibiofilm activity by screening HMOs from 14 new donors against three strains of GBS and two of the ESKAPE pathogens of particular interest to child health, Staphylococcus aureus and Acinetobacter baumannii. Growth and biofilm assays showed that HMOs from these new donors possessed antimicrobial and antibiofilm activity against all three strains of GBS, antibiofilm activity against methicillin-resistant S. aureus strain USA300, and antimicrobial activity against A. baumannii strain ATCC 19606.