Effect of Chronic Kidney Disease on Changes in Vasopressin System Expression in the Kidney Cortex in Rats with Nephrectomy

It is believed that the vasopressinergic system plays an important role in the pathogenesis of chronic kidney disease (CKD). The aim of this study was to evaluate the effect of CKD on changes in vasopressin system expression in the kidney cortex in rats with nephrectomy. The study was performed on 4...

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Published in:BioMed research international 2018-01, Vol.2018 (2018), p.1-10
Main Authors: Niemczyk, Stanisław, Zmudzki, Wawrzyniec, Puchalska, L., Tkaczyk, Marzanna, Wrzesien, Robert, Niemczyk, Longin, Cudnoch-Jedrzejewska, A., Czarzasta, K., Saracyn, Marek
Format: Article
Language:English
Subjects:
Animals
Aprotinin
Aquaporin 2
Aquaporins
Argipressin receptors
Chronic kidney failure
Ethylenediaminetetraacetic acid
Gene expression
Heart failure
Kidney
Kidney Cortex - metabolism
Kidney diseases
Kidneys
Laboratories
Nephrectomy
Neurophysiology
Pathogenesis
Physiology
Plasma
Proteins
Rats
Rats, Sprague-Dawley
Receptors
Receptors, Vasopressin
Renal cortex
Renal Insufficiency, Chronic - physiopathology
RNA
Rodents
Type 2 diabetes
Urine
Vasopressin
Vasopressin receptors
Vasopressin V2 receptors
Vasopressins - metabolism
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Summary:It is believed that the vasopressinergic system plays an important role in the pathogenesis of chronic kidney disease (CKD). The aim of this study was to evaluate the effect of CKD on changes in vasopressin system expression in the kidney cortex in rats with nephrectomy. The study was performed on 4 groups of Sprague Dawley (SPRD) rats: a control group (CN), 1/2 nephrectomy (N1/2), 2/3 nephrectomy (N2/3), and 5/6 nephrectomy (N5/6). Blood and the kidney cortex were collected to evaluate plasma copeptin concentrations and mRNA expressions of V1a vasopressin receptors (V1aR) and V2 vasopressin receptors (V2R) and V1aR, V2R, and aquaporin 2 (AQP2) protein levels. V1aR and V2R mRNA expression in the kidney cortex was significantly lower in the CN group compared with the other groups. In contrast, the V1aR, V2R, and AQP2 protein levels were significantly higher in the CN group compared with all of the nephrectomized groups. Plasma copeptin concentration was significantly lower in the CN group than in the nephrectomized groups. CKD caused significant changes in the expression of the vasopressinergic system. Further research is needed to explain the mechanisms of the impact of the vasopressinergic system on the kidney in CKD.
ISSN:2314-6133
2314-6141
DOI:10.1155/2018/2607928