Development of T3SS Mutants (hrpB− and hrcV−) of Ralstonia solanacearum, Evaluation of Virulence Attenuation in Brinjal and Tomato—A Pre-requisite to Validate T3Es of R. solanacearum

Ralstonia solanacearum is an important plant pathogen which infects a large number of agriculturally important crops. The Type Three Secretion System (T3SS) plays a major role in its pathogenicity by secreting type III effectors (T3Es) which overthrow the host defence mechanism. The secretion of T3E...

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Bibliographic Details
Published in:Indian journal of microbiology 2018-09, Vol.58 (3), p.372-380
Main Authors: Asolkar, Trupti, Ramesh, Raman
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
crops
eggplants
fimbriae
genes
Homologous recombination
Homology
hosts
in vitro studies
Inoculation
Insertional mutagenesis
Life Sciences
Medical Microbiology
Microbiology
Mutants
Original
Original Research Article
Pathogenicity
Pathogens
petioles
plant pathogens
plasmids
Ralstonia solanacearum
Secretion
soil drenching
Tomatoes
Transcription
transcription (genetics)
Translocation
type III secretion system
Vascular system (plant anatomy)
Virulence
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Summary:Ralstonia solanacearum is an important plant pathogen which infects a large number of agriculturally important crops. The Type Three Secretion System (T3SS) plays a major role in its pathogenicity by secreting type III effectors (T3Es) which overthrow the host defence mechanism. The secretion of T3Es is transcriptionally regulated by hrpB and its secretion is dependent on the pili formed by hrcV gene. In this study, two T3SS mutants of R. solanacearum strain Rs-09-161 viz. Rs-HrpB − and Rs-HrcV − were developed through insertional mutagenesis. The method of development of insertional mutant is quite simple and reliable. The plasmid integrates through homologous recombination and in vitro studies have proved that the integration was stable for several generations. The mutants are non-pathogenic on its highly susceptible hosts, brinjal and tomato inoculated by soil drench method and by petiole inoculation directly into the vascular system. Further it was observed that the colonisation ability of the mutants was also highly reduced in the susceptible host. These mutants will be useful in validating putative T3E through translocation studies.
ISSN:0046-8991
0973-7715
DOI:10.1007/s12088-018-0736-y