Carnosic acid impedes cell growth and enhances anticancer effects of carmustine and lomustine in melanoma

Carnosic acid (CA), a major polyphenolic diterpene present in , has been reported to have multiple functions, including antitumor activity. The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was use...

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Bibliographic Details
Published in:Bioscience reports 2018-08, Vol.38 (4)
Main Authors: Lin, Kun-I, Lin, Chih-Chien, Kuo, Shyh-Ming, Lai, Jui-Chi, Wang, You-Qi, You, Huey-Ling, Hsu, Mei-Ling, Chen, Chang-Han, Shiu, Li-Yen
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents, Alkylating - pharmacology
Antineoplastic Agents, Alkylating - therapeutic use
Antioxidants - pharmacology
Antioxidants - therapeutic use
Apoptosis - drug effects
Carmustine - pharmacology
Carmustine - therapeutic use
Cell Cycle Checkpoints - drug effects
Cell Line, Tumor
Cell Proliferation - drug effects
Diterpenes, Abietane - pharmacology
Diterpenes, Abietane - therapeutic use
Drug Synergism
Lomustine - pharmacology
Lomustine - therapeutic use
Male
Melanoma, Experimental - drug therapy
Melanoma, Experimental - pathology
Mice, Inbred C57BL
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Summary:Carnosic acid (CA), a major polyphenolic diterpene present in , has been reported to have multiple functions, including antitumor activity. The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was used for cell cycle detection. p21 and p27 expression was detected by Western blotting. B16F10 cell xenograft model was established, and treated with CA, carmustine (BCNU), or lomustine (CCNU). The present study found that CA exhibits significant growth inhibition and cell cycle arrest in melanoma B16F10 cells. We also found that CA triggers cell cycle arrest at G /G phase, and enhances p21 expression. Additionally, CA can enhance BCNU- and CCNU-mediated cytotoxicity and cell cycle arrest in B16F10 cells. Finally, we found that CA inhibits tumor growth, and reduces the values of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) The present study study concluded that CA may be safe and useful as a novel chemotherapeutic agent.
ISSN:0144-8463
1573-4935
DOI:10.1042/bsr20180005