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Carnosic acid impedes cell growth and enhances anticancer effects of carmustine and lomustine in melanoma
Carnosic acid (CA), a major polyphenolic diterpene present in , has been reported to have multiple functions, including antitumor activity. The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was use...
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| Published in: | Bioscience reports 2018-08, Vol.38 (4) |
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| Main Authors: | , , , , , , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c447t-fc5d7e229d752b8e10c8d5f5d9eb123bdef5cf782237925201e097e57052edc53 |
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| cites | cdi_FETCH-LOGICAL-c447t-fc5d7e229d752b8e10c8d5f5d9eb123bdef5cf782237925201e097e57052edc53 |
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| container_issue | 4 |
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| container_title | Bioscience reports |
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| creator | Lin, Kun-I Lin, Chih-Chien Kuo, Shyh-Ming Lai, Jui-Chi Wang, You-Qi You, Huey-Ling Hsu, Mei-Ling Chen, Chang-Han Shiu, Li-Yen |
| description | Carnosic acid (CA), a major polyphenolic diterpene present in
, has been reported to have multiple functions, including antitumor activity. The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was used for cell cycle detection. p21 and p27 expression was detected by Western blotting. B16F10 cell xenograft model was established, and treated with CA, carmustine (BCNU), or lomustine (CCNU). The present study found that CA exhibits significant growth inhibition and cell cycle arrest in melanoma B16F10 cells. We also found that CA triggers cell cycle arrest at G
/G
phase, and enhances p21 expression. Additionally, CA can enhance BCNU- and CCNU-mediated cytotoxicity and cell cycle arrest in B16F10 cells. Finally, we found that CA inhibits tumor growth, and reduces the values of aspartate aminotransferase (AST) and alanine aminotransferase (ALT)
The present study study concluded that CA may be safe and useful as a novel chemotherapeutic agent. |
| doi_str_mv | 10.1042/bsr20180005 |
| format | article |
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, has been reported to have multiple functions, including antitumor activity. The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was used for cell cycle detection. p21 and p27 expression was detected by Western blotting. B16F10 cell xenograft model was established, and treated with CA, carmustine (BCNU), or lomustine (CCNU). The present study found that CA exhibits significant growth inhibition and cell cycle arrest in melanoma B16F10 cells. We also found that CA triggers cell cycle arrest at G
/G
phase, and enhances p21 expression. Additionally, CA can enhance BCNU- and CCNU-mediated cytotoxicity and cell cycle arrest in B16F10 cells. Finally, we found that CA inhibits tumor growth, and reduces the values of aspartate aminotransferase (AST) and alanine aminotransferase (ALT)
The present study study concluded that CA may be safe and useful as a novel chemotherapeutic agent.</description><identifier>ISSN: 0144-8463</identifier><identifier>EISSN: 1573-4935</identifier><identifier>DOI: 10.1042/bsr20180005</identifier><identifier>PMID: 29789400</identifier><language>eng</language><publisher>England: Portland Press Ltd</publisher><subject>Animals ; Antineoplastic Agents, Alkylating - pharmacology ; Antineoplastic Agents, Alkylating - therapeutic use ; Antioxidants - pharmacology ; Antioxidants - therapeutic use ; Apoptosis - drug effects ; Carmustine - pharmacology ; Carmustine - therapeutic use ; Cell Cycle Checkpoints - drug effects ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Diterpenes, Abietane - pharmacology ; Diterpenes, Abietane - therapeutic use ; Drug Synergism ; Lomustine - pharmacology ; Lomustine - therapeutic use ; Male ; Melanoma, Experimental - drug therapy ; Melanoma, Experimental - pathology ; Mice, Inbred C57BL</subject><ispartof>Bioscience reports, 2018-08, Vol.38 (4)</ispartof><rights>2018 The Author(s).</rights><rights>2018 The Author(s). 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-fc5d7e229d752b8e10c8d5f5d9eb123bdef5cf782237925201e097e57052edc53</citedby><cites>FETCH-LOGICAL-c447t-fc5d7e229d752b8e10c8d5f5d9eb123bdef5cf782237925201e097e57052edc53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028752/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028752/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29789400$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Kun-I</creatorcontrib><creatorcontrib>Lin, Chih-Chien</creatorcontrib><creatorcontrib>Kuo, Shyh-Ming</creatorcontrib><creatorcontrib>Lai, Jui-Chi</creatorcontrib><creatorcontrib>Wang, You-Qi</creatorcontrib><creatorcontrib>You, Huey-Ling</creatorcontrib><creatorcontrib>Hsu, Mei-Ling</creatorcontrib><creatorcontrib>Chen, Chang-Han</creatorcontrib><creatorcontrib>Shiu, Li-Yen</creatorcontrib><title>Carnosic acid impedes cell growth and enhances anticancer effects of carmustine and lomustine in melanoma</title><title>Bioscience reports</title><addtitle>Biosci Rep</addtitle><description>Carnosic acid (CA), a major polyphenolic diterpene present in
, has been reported to have multiple functions, including antitumor activity. The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was used for cell cycle detection. p21 and p27 expression was detected by Western blotting. B16F10 cell xenograft model was established, and treated with CA, carmustine (BCNU), or lomustine (CCNU). The present study found that CA exhibits significant growth inhibition and cell cycle arrest in melanoma B16F10 cells. We also found that CA triggers cell cycle arrest at G
/G
phase, and enhances p21 expression. Additionally, CA can enhance BCNU- and CCNU-mediated cytotoxicity and cell cycle arrest in B16F10 cells. Finally, we found that CA inhibits tumor growth, and reduces the values of aspartate aminotransferase (AST) and alanine aminotransferase (ALT)
The present study study concluded that CA may be safe and useful as a novel chemotherapeutic agent.</description><subject>Animals</subject><subject>Antineoplastic Agents, Alkylating - pharmacology</subject><subject>Antineoplastic Agents, Alkylating - therapeutic use</subject><subject>Antioxidants - pharmacology</subject><subject>Antioxidants - therapeutic use</subject><subject>Apoptosis - drug effects</subject><subject>Carmustine - pharmacology</subject><subject>Carmustine - therapeutic use</subject><subject>Cell Cycle Checkpoints - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Diterpenes, Abietane - pharmacology</subject><subject>Diterpenes, Abietane - therapeutic use</subject><subject>Drug Synergism</subject><subject>Lomustine - pharmacology</subject><subject>Lomustine - therapeutic use</subject><subject>Male</subject><subject>Melanoma, Experimental - drug therapy</subject><subject>Melanoma, Experimental - pathology</subject><subject>Mice, Inbred C57BL</subject><issn>0144-8463</issn><issn>1573-4935</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpVkc1PGzEQxS1UVALtiXvlYyW04M9491KpRHxUQkKC9mx57XHiatdO7U0R_z1OSVA52Z7305sZP4ROKTmnRLCLvmRGaEsIkQdoRqXijei4_IBmhArRtGLOj9BxKb8rUQXxER2xTrWdIGSGwsLkmEqw2NjgcBjX4KBgC8OAlzk9TStsosMQVybaKpg4Bbu9Zgzeg50KTh5bk8dNmUKEf_SQ9q8Q8QiDiWk0n9ChN0OBz7vzBP26vvq5uG3u7m9-LL7fNVYINTXeSqeAsc4pyfoWKLGtk166DnrKeO_AS-tVyxhXHZN1cyCdAqmIZOCs5Cfo26vvetOPtQJxymbQ6xxGk591MkG_V2JY6WX6q-eEtbVnNfi6M8jpzwbKpMdQth9iIqRN0YwITueKcVrRs1fU5lRKBv_WhhK9DUdfPj7sw6n0l_8ne2P3afAXEjSMig</recordid><startdate>20180831</startdate><enddate>20180831</enddate><creator>Lin, Kun-I</creator><creator>Lin, Chih-Chien</creator><creator>Kuo, Shyh-Ming</creator><creator>Lai, Jui-Chi</creator><creator>Wang, You-Qi</creator><creator>You, Huey-Ling</creator><creator>Hsu, Mei-Ling</creator><creator>Chen, Chang-Han</creator><creator>Shiu, Li-Yen</creator><general>Portland Press Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180831</creationdate><title>Carnosic acid impedes cell growth and enhances anticancer effects of carmustine and lomustine in melanoma</title><author>Lin, Kun-I ; Lin, Chih-Chien ; Kuo, Shyh-Ming ; Lai, Jui-Chi ; Wang, You-Qi ; You, Huey-Ling ; Hsu, Mei-Ling ; Chen, Chang-Han ; Shiu, Li-Yen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-fc5d7e229d752b8e10c8d5f5d9eb123bdef5cf782237925201e097e57052edc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Antineoplastic Agents, Alkylating - pharmacology</topic><topic>Antineoplastic Agents, Alkylating - therapeutic use</topic><topic>Antioxidants - pharmacology</topic><topic>Antioxidants - therapeutic use</topic><topic>Apoptosis - drug effects</topic><topic>Carmustine - pharmacology</topic><topic>Carmustine - therapeutic use</topic><topic>Cell Cycle Checkpoints - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Diterpenes, Abietane - pharmacology</topic><topic>Diterpenes, Abietane - therapeutic use</topic><topic>Drug Synergism</topic><topic>Lomustine - pharmacology</topic><topic>Lomustine - therapeutic use</topic><topic>Male</topic><topic>Melanoma, Experimental - drug therapy</topic><topic>Melanoma, Experimental - pathology</topic><topic>Mice, Inbred C57BL</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Kun-I</creatorcontrib><creatorcontrib>Lin, Chih-Chien</creatorcontrib><creatorcontrib>Kuo, Shyh-Ming</creatorcontrib><creatorcontrib>Lai, Jui-Chi</creatorcontrib><creatorcontrib>Wang, You-Qi</creatorcontrib><creatorcontrib>You, Huey-Ling</creatorcontrib><creatorcontrib>Hsu, Mei-Ling</creatorcontrib><creatorcontrib>Chen, Chang-Han</creatorcontrib><creatorcontrib>Shiu, Li-Yen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Bioscience reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Kun-I</au><au>Lin, Chih-Chien</au><au>Kuo, Shyh-Ming</au><au>Lai, Jui-Chi</au><au>Wang, You-Qi</au><au>You, Huey-Ling</au><au>Hsu, Mei-Ling</au><au>Chen, Chang-Han</au><au>Shiu, Li-Yen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carnosic acid impedes cell growth and enhances anticancer effects of carmustine and lomustine in melanoma</atitle><jtitle>Bioscience reports</jtitle><addtitle>Biosci Rep</addtitle><date>2018-08-31</date><risdate>2018</risdate><volume>38</volume><issue>4</issue><issn>0144-8463</issn><eissn>1573-4935</eissn><abstract>Carnosic acid (CA), a major polyphenolic diterpene present in
, has been reported to have multiple functions, including antitumor activity. The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was used for cell cycle detection. p21 and p27 expression was detected by Western blotting. B16F10 cell xenograft model was established, and treated with CA, carmustine (BCNU), or lomustine (CCNU). The present study found that CA exhibits significant growth inhibition and cell cycle arrest in melanoma B16F10 cells. We also found that CA triggers cell cycle arrest at G
/G
phase, and enhances p21 expression. Additionally, CA can enhance BCNU- and CCNU-mediated cytotoxicity and cell cycle arrest in B16F10 cells. Finally, we found that CA inhibits tumor growth, and reduces the values of aspartate aminotransferase (AST) and alanine aminotransferase (ALT)
The present study study concluded that CA may be safe and useful as a novel chemotherapeutic agent.</abstract><cop>England</cop><pub>Portland Press Ltd</pub><pmid>29789400</pmid><doi>10.1042/bsr20180005</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Bioscience reports, 2018-08, Vol.38 (4) |
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| source | PubMed Central |
| subjects | Animals Antineoplastic Agents, Alkylating - pharmacology Antineoplastic Agents, Alkylating - therapeutic use Antioxidants - pharmacology Antioxidants - therapeutic use Apoptosis - drug effects Carmustine - pharmacology Carmustine - therapeutic use Cell Cycle Checkpoints - drug effects Cell Line, Tumor Cell Proliferation - drug effects Diterpenes, Abietane - pharmacology Diterpenes, Abietane - therapeutic use Drug Synergism Lomustine - pharmacology Lomustine - therapeutic use Male Melanoma, Experimental - drug therapy Melanoma, Experimental - pathology Mice, Inbred C57BL |
| title | Carnosic acid impedes cell growth and enhances anticancer effects of carmustine and lomustine in melanoma |
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