Nanoparticle-Mediated Cell Capture Enables Rapid Endothelialization of a Novel Bare Metal Stent

Incomplete endothelialization of intracoronary stents has been associated with stent thrombosis and recurrent symptoms, whereas prolonged use of dual antiplatelet therapy increases bleeding-related adverse events. Facilitated endothelialization has the potential to improve clinical outcomes in patie...

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Bibliographic Details
Published in:Tissue engineering. Part A 2018-07, Vol.24 (13-14), p.1157-1166
Main Authors: Tefft, Brandon J., Uthamaraj, Susheil, Harbuzariu, Adriana, Harburn, J. Jonathan, Witt, Tyra A., Newman, Brant, Psaltis, Peter J., Hlinomaz, Ota, Holmes, David R., Gulati, Rajiv, Simari, Robert D., Dragomir-Daescu, Dan, Sandhu, Gurpreet S.
Format: Article
Language:English
Subjects:
Animals
Antiplatelet therapy
Biocompatibility
Biodegradability
Biomedical materials
Cell culture
Coronary artery
Drug therapy
Endothelial cells
Endothelial Cells - cytology
Endothelial Cells - drug effects
Endothelial Cells - ultrastructure
Female
Glycolic acid
Implants
Iron
Iron oxides
Magnetite
Medicine
Metals - chemistry
Nanoparticles
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Nanotechnology
Original
Original Articles
Phenotype
Polyethylene glycol
Stainless steel
Stainless Steel - pharmacology
Stents
Swine
Thrombosis
Transplants & implants
Veins & arteries
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Summary:Incomplete endothelialization of intracoronary stents has been associated with stent thrombosis and recurrent symptoms, whereas prolonged use of dual antiplatelet therapy increases bleeding-related adverse events. Facilitated endothelialization has the potential to improve clinical outcomes in patients who are unable to tolerate dual antiplatelet therapy. The objective of this study was to demonstrate the feasibility of magnetic cell capture to rapidly endothelialize intracoronary stents in a large animal model. A novel stent was developed from a magnetizable duplex stainless steel (2205 SS). Polylactic-co-glycolic acid and magnetite (Fe 3 O 4 ) were used to synthesize biodegradable superparamagnetic iron oxide nanoparticles, and these were used to label autologous blood outgrowth endothelial cells. Magnetic 2205 SS and nonmagnetic 316L SS control stents were implanted in the coronary arteries of pigs ( n  = 11), followed by intracoronary delivery of magnetically labeled cells to 2205 SS stents. In this study, we show extensive endothelialization of magnetic 2205 SS stents (median 98.4% cell coverage) within 3 days, whereas the control 316L SS stents exhibited significantly less coverage (median 48.9% cell coverage, p  
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2017.0404