Astrocyte-specific transcriptome responses to chronic ethanol consumption

Astrocytes play critical roles in central nervous system (CNS) homeostasis and are implicated in the pathogenesis of neurological and psychiatric conditions, including drug dependence. Little is known about the effects of chronic ethanol consumption on astrocyte gene expression. To address this gap...

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Bibliographic Details
Published in:The pharmacogenomics journal 2018-07, Vol.18 (4), p.578-589
Main Authors: Erickson, Emma K., Farris, Sean P., Blednov, Yuri A., Mayfield, R. Dayne, Harris, R. Adron
Format: Article
Language:English
Subjects:
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Alcohol metabolism
Astrocytes
Biochemistry
Biomedical and Life Sciences
Biomedicine
Calcium
Calcium signalling
Central nervous system
Dependence
Drinking (Alcoholic beverages)
Drug dependence
Ethanol
Extracellular matrix
Gene Expression
Gene regulation
Genes
Genetic aspects
Health aspects
Homeostasis
Human Genetics
Oncology
Pharmacotherapy
Prefrontal cortex
Psychopharmacology
Ribonucleic acid
RNA
RNA sequencing
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Summary:Astrocytes play critical roles in central nervous system (CNS) homeostasis and are implicated in the pathogenesis of neurological and psychiatric conditions, including drug dependence. Little is known about the effects of chronic ethanol consumption on astrocyte gene expression. To address this gap in knowledge, we performed transcriptome-wide RNA sequencing of astrocytes isolated from the prefrontal cortex (PFC) of mice following chronic ethanol consumption. Differential expression analysis revealed ethanol-induced changes unique to astrocytes that were not identified in total homogenate preparations. Astrocyte-specific gene expression revealed calcium-related signaling and regulation of extracellular matrix genes as responses to chronic ethanol use. These findings emphasize the importance of investigating expression changes in specific cellular populations to define molecular consequences of chronic ethanol consumption in mammalian brain.
ISSN:1470-269X
1473-1150
DOI:10.1038/s41397-017-0012-2